数字中医药（英文）2020，Vol.3Issue(2)：57-66,10.DOI:10.1016/j.dcmed.2020.06.001
中药金百美颜方对中波紫外诱导HaCaT细胞氧化损伤和光老化的保护作用
Protective Effects of Jin Bai Mei Yan Prescription on Oxidative Damage and Photoaging Induced by Ultraviolet B in HaCaT Cells
摘要
Abstract
Objective Ultraviolet B (UVB) mainly acts on the skin epidermis, causing oxidative damage and apoptosis of keratinocytes. Jin Bai Mei Yan Prescription (JBMYP) comprises a variety of antioxidant traditional Chinese medicines (TCM). In this study, we aimed to evaluate the effects of JBMYP on the oxidative damage induced by UVB in human immortalized epidermal keratinocytes (HaCaT) cells.Methods HaCaT cells were divided into six groups: control group, model (UVB) group, positive (UVB + vitamin E) group, UVB +JBMYP low dose group (160 μg/mL), UVB +JBMYP mode-rate dose group (800 μg/mL), and UVB +JBMYP high dose group (1600 μg/mL). HaCaT cells were irradiated with UVB and treated with JBMYP for 24 h. Methyl thiazolyl tetrazolium (MTT) assay and real-time unlabeled cell function analyzer were used to assess the cell survival and proliferation rates, respectively. At the same time, the levels of intracellular reactive oxygen species (ROS), lipid peroxide malondialdehyde (MDA), glutathione (GSH), and hydroxyproline (HYP), as well as the activities of antioxidant enzyme superoxide dismutase (SOD) and catalase (CAT) were evaluated using enzyme linked immunosorbent assay (ELISA). Results Compared with the model group, the survival rate of HaCaT cells in each dosage group of JBMYP was significantly improved (P < 0.05). Further, JBMYP could promote the proliferation of HaCaT cells, leading to a reduction in the contents of MDA and ROS, and increase in the contents of SOD, CAT, GSH and HYP in HaCaT cells. Conclusions JBMYP has enhanced protective effect on oxidative damage induced by UVB in HaCaT cells.关键词
金百美颜方/HaCaT/氧化损伤/UVB/中药/抗衰老Key words
Jin Bai Mei Yan Prescription (JBMYP)/HaCaT cell/Oxidative damage/Ultraviolet B (UVB)/Chinese medicine/Anti-aging引用本文复制引用
NORIKO Minamisawa,苗明三,康乐,刘慧娟,崔琳琳,HIROKI Kanemisu,TERUMI Naito,HIROAKI Shinotsuka..中药金百美颜方对中波紫外诱导HaCaT细胞氧化损伤和光老化的保护作用[J].数字中医药（英文）,2020,3(2):57-66,10.基金项目
We thank for the funding support from the National Administration of Traditional Chinese Medicine (No. 2017-149-11), National Base for International Cooperation (No. 2016-65) and Henan Province Industry-University-Research Collaboration (No.182107000029) to conduct the Special Project on Standardization of Traditional Chinese Medicine. （No. 2017-149-11）
