数字中医药（英文）2020，Vol.3Issue(2)：116-132,17.DOI:10.1016/j.dcmed.2020.06.005
基于网络药理学探讨湖南省专家组推荐的新型冠状病毒肺炎中药预防用方二的预防机制
Network Pharmacology Approach to Investigate the Preventive Mechanism of Hunan Expert Group Recommended Chinese Medicine Prevention No. 2 Prescription Against COVID-19
摘要
Abstract
Objective To explore the possible preventive mechanism of Hunan expert group recommended Chinese medicine prescription of No. 2 (Pre-No. 2) against coronavirus disease 2019 (COVID-19) by network pharmacology method. Methods The target proteins of effective components and active compounds in Pre-No. 2 were screened by searching the Tradi-tional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). A component-target-disease interac-tion network of Pre-No. 2 was constructed by Cytoscape 3.7.2, gene ontology (GO) analysis, and Kyoto encyclopedia of genes and genomes (KEGG) analysis of target protein pathway by DAVID. Results A total of 163compounds and 278 target protein targets in Pre-No. 2 were collected from the TCMSP database. Kaempferol, wogonin, 7-methoxy-2-methyl isoflavone, formononetin, isorhamnetin, and licochalcone A were the most frequent targets in the regulatory network. GO enrichment analysis showed that Pre-No. 2 regulated response to virus, viral processes, humoral immune responses, defense responses to virus and viral entry into host cells. KEGG enrichment analysis showed that the formula regulated the NF-κB signaling pathway, B cell receptor signaling pathway, viral carcinogenesis, T cell signaling pathway and FcγR-mediated phagocytosis signaling pathway. Conclusions Pre-No. 2 may play a preventive role against COVID-19 through regulation of the Toll-like signaling, T cell signaling, B cell signaling and other signaling pathways. It may re-gulate the immune system to protect against anti-influenza virus.关键词
湖南省专家组推荐新型冠状病毒肺炎中药预防用方二/新型冠状病毒肺炎/网络药理学/免疫系统Key words
Hunan expert group recommended Chinesemedicine prevention No. 2 prescription (Pre-No. 2)/Coronavirus disease 2019 (COVID-19)/Network pharmacology/Immune system引用本文复制引用
吴华英,李静,毛一之,袁志鹰,LI Feng,李亮,黄惠勇..基于网络药理学探讨湖南省专家组推荐的新型冠状病毒肺炎中药预防用方二的预防机制[J].数字中医药（英文）,2020,3(2):116-132,17.基金项目
We thank for the funding support from the Scientific Research Fund of Hunan Administration of TCM (No. KYGG06 and No. KYGG07). （No. KYGG06 and No. KYGG07）
