生命科学研究2020，Vol.24Issue(3)：187-198,12.DOI:10.16605/j.cnki.1007-7847.2020.03.003
中国江苏太湖和阳澄湖细菌群落组成、多样性和时空动态比较研究
Comparison of the Composition, Diversity and Spatiotemporal Dynamics of Bacterial Communities in Lake Taihu and Lake Yangcheng, Jiangsu, China
摘要
Abstract
To investigate and compare the distribution,diversity and dynamics of bacterioplankton communities in Lake Taihu and Lake Yangcheng in Jiangsu Province,China,the bacterial community composition was analyzed from 85 samples using 454-pyrosequencing of 16S rRNA genes.The samples were taken weekly within an intensive time period from June to October 2012.A total of 142 354 clean reads were generated that were assigned to 4 589 operational taxonomic units (OTUs),with Proteobacteria (mainly γ-Proteobacteria) and Bacteroidetes being the dominant taxa in both lakes.In addition,19.55％ of OTUs,accounting for 95.01％ of the total reads,were shared by bacterial communities in the two lakes,which indicated a high degree of overlap between them.The communities in Lake Yangcheng had a higher α-diversity than those in Lake Taihu,which may be mainly attributed to the rare taxa found in both lakes.Interestingly,the results showed that the bacterioplankton composition profile dramatically fluctuated over time.This may be explained by the low capability of the communities to resist external disturbances in extremely shallow lakes.Our study provides information for better understanding of the spatiotemporal dynamics in bacterial communities and differences in their compositions between two related lakes.关键词
细菌群落/16S rRNA基因/454焦磷酸测序/太湖/阳澄湖Key words
bacterial community/16S rRNA gene/454-pyrosequencing/Lake Taihu/Lake Yangcheng分类
生物科学引用本文复制引用
李向阳,杨紫琳..中国江苏太湖和阳澄湖细菌群落组成、多样性和时空动态比较研究[J].生命科学研究,2020,24(3):187-198,12.基金项目
国家自然科学基金资助项目(21507012,21966015) （21507012,21966015）
中国博士后科学基金资助项目(2015M570329) （2015M570329）
贵州省科学技术基金资助项目(黔科合基础[2019]1287号) （黔科合基础[2019]1287号）
贵州省科技厅联合基金资助项目(黔科合LH字[2017]7176) The National Natural Science Foundation of China (21507012,21966015) （黔科合LH字[2017]7176）
China Postdoctoral Science Foundation (2015M570329) （2015M570329）
the Science and Technology Foundation of Guizhou Province of China (QKHJC[2019]1287) and Joint Foundation of Guizhou Province Science and Technology Commission of China (LH[2017]7176) （QKHJC[2019]1287）
