中国药房2020，Vol.31Issue(14)：1683-1690,8.DOI:10.6039/j.issn.1001-0408.2020.14.04
苗药红禾麻提取物在离体人肠道菌群中的代谢研究
Study on Metabolism of Miao Medicine Laportea bulbifera Extract in Isolated Human Intestinal Flora
摘要
Abstract
OBJECTIVE: To explore the metabolic characteristics of Miao medicine Laportea bulbifera extract in isolated human intestinal flora. METHODS: L. bulbifera was extracted with 70% ethanol reflux extraction. After concentration. Extraction with n-butanol and drying, L. bulbifera was extracted obtained. Taking 0.05 g/mL L. bulbifera extract 1 mL mixed with isolated human intestinal flora fluid 10 mL and cultured for 36 h in anaerobic environment (setting up blank control without drugs or human intestinal bacterial solution), so as to simulate the metabolic process of the extract in human intestine. The metabolites were detected by UPLC-Q-TOF/MS. The determination was performed on Agilent Eclipse Plus C18 RRHD column with mobile phase consisted solution of 0.01% formic acid water solution-0.01% formic acid acetonitrile solution (gradient eluetion) at the flow rate of 0.25 mL/min. The column temperature was set at 40℃，and the sample size was 1μL. ESI detection was adopted and scanned by negative ion mode (ESI-); the capillary voltage was4.5kV, the ion source temperature was 120℃, the collision energy was 15-32 V ,and the scanning range was m/z 50-1000.The "Strip" module of MassLynx V4.1 software was used to analyze differential chromatograms between the reaction solution and the blank control of L.bulbifera extract. Mass spectrum data and UNIFI software were used to predict relative molecular weight and formula; based on the information of substance control and related literature reports, the structure and biotransformation pathway of L.bulbfera metabolites in isolated human intestinal flora were predicted and analyzed. RESULTS & CONCLUSIONS:A total of 3 prototype products (rutin, quercetin, kaempferol-3-O-rutinoside) and 22 metabolites (mainly the metabolites of quercetin, mono-caffeoylquinic acid, isoquercitrin, etc.) were detected after metabolized in isolated human intestinal flora. Its biotransformation pathway is phase I reaction, which mainly consisted of reduction, oxidation and hydrolysis.关键词
红禾麻/离体人肠道菌群/代谢/超高效液相色谱-四极杆-飞行时间质谱联用技术/鉴定Key words
Laportea bulbifera/Isolated human intestinal flora/Metabolism/UPLC-Q-TOF/MS/Identification分类
医药卫生引用本文复制引用
薛寸,王永林,吴耽,巩仔鹏,陈思颖,唐娟,李月婷,王爱民,李勇军,兰燕宇..苗药红禾麻提取物在离体人肠道菌群中的代谢研究[J].中国药房,2020,31(14):1683-1690,8.基金项目
国家自然科学基金资助项目(No.81560693,No.U1812403) （No.81560693,No.U1812403）
中央引导地方科技发展专项资金项目(No.黔科中引地〔2018〕4006) （No.黔科中引地〔2018〕4006）
贵州省民族药药效物质基础研究科技创新人才团队项目(No.黔科合平台人才〔2016〕5613) （No.黔科合平台人才〔2016〕5613）
贵州省高层次创新型人才培养计划项目(No.黔科合平台人才〔2016〕5677) （No.黔科合平台人才〔2016〕5677）
贵阳市科技计划项目(No.筑科合同〔2017〕30-29号) （No.筑科合同〔2017〕30-29号）
