摘要
Abstract
Objective: To investigate the effect and its molecular mechanism of micro RNA-541-3p (miR-541-3p) on the proliferation,migration,and invasion of cutaneous melanoma cells.Methods:qRT-PCR and Western blotting were used to detect the expression levels of miR-541-3p and SENP1 in skin melanoma tissues.Taking human skin melanoma cells A375 as the research object,miR-NC,miR-541-3p mimics,si-NC,siSENP1,miR-541-3p mimics,and pcDNA,miR-541-3p mimics and pcDNA-SENP1 were transfected into A375 cells.MTT was used to detect cell proliferation.Transwell chamber experiment was used to detect cell migration and invasion ability.The double luciferase reporting experiment was used to verifythe effect of miR-541-3p overexpression on wild-type and mutant SENP1 luciferase activity.Western blotting was used to detect the protein expression of CyclinD1,p21,MMP-2,and MMP-9.Results:Compared with normal skin tissue,the expression of miR-541-3p in skin melanoma tissue was significantly decreased (P＜0.05),and the expression levels of SENP1 mRNA and protein were significantly increased (P＜0.05).Compared with the miR-NC group,in the miR-541-3p group,the cellular viability was significantly decreased (P＜0.05),and the number of migrating cells and invasive cells were significantly decreased (P＜0.05);the proteins levels of CyclinD1,MMP-2,and MMP-9 were significantly decreased (P＜0.05),and the protein level of p21 was significantly increased (P＜0.05).Compared with the si-NC group,in the siSENP1 group,the cellular viability was significantly decreased (P＜0.05),the number of migrating cells and invasive cells weresignificantly decreased (P＜0.05),and the protein levels of CyclinD1,MMP-2,and MMP-9 were decreased significantly (P＜0.05),and the protein level of p21 was increased significantly (P＜0.05).The double luciferase reporting experiment confirmed that miR-541-3p could inhibit the luciferase activity in the 3'-UTR region of SENP 1.Compared with the miR-541-3p + pcDNA group,in the miR-541-3p + pcDNA-SENP1 group,the cell viability was significantly increased (P＜0.05),and the number of migrating cells and invasive cells weresignificantly increased (P＜0.05),the protein levels of CyclinD1,MMP-2,and MMP-9 were increased significantly (P＜0.05),the protein levels of p21 was decreased significantly (P＜0.05).Conclusion: Overexpression of miR-541-3p can inhibit the expression of SENP1 and attenuate proliferation,migration,and invasion ability of skin melanoma cell.关键词
miR-541-3p/SENP1/皮肤黑色素瘤/增殖/迁移/侵袭Key words
miR-541-3p/SENP1/cutaneous melanoma/proliferation/migration/invasion分类
医药卫生
