广西医科大学学报2020，Vol.37Issue(8)：1389-1397,9.DOI:10.16190/j.cnki.45-1211/r.2020.08.001
miR-28-3p通过PI3K/AKT通路对大鼠糖尿病性视网膜病变及视网膜血管生成的影响
Effect of miR-28-3p via PI3K/AKT pathway on diabetic retinopathy and retinal angiogenesis in rats
摘要
Abstract
Objective:To investigate the effect of miR-28-3p on of diabetic retinopathy(DR) and retina angiogenesis in rats by PI3K/AKT pathway.Methods: SD rats were divided into sham operation group (sham group),diabetes model group (model group),diabetes + negative control group (miR-NC group) and diabetes+miR-28-3p inhibitor group (miR-28-3p inhibitor group),the diabetic rat model was established by intraperitoneal injection of streptozotocin solution and was treated with adeno-associated virus (AAV-U6-inhibitor-rno-miR-28-3p-CAG-EGFP).qPCR was used to detect the expression of miR-28-3p in retinal tissue of each group.HE staining was used to observe the distribution of blood vessels in the retina of each group.Immunofluorescence was used to detect the expression of CD31.The expression of Bax and Bcl-2 protein in retinal tissue of each group was detected by immunohistochemistry.The expression of inflammatory factors (TNF-α,IL-1β and IL-6) in serum of each group was detected by ELISA.The expression of PI3K and AKT protein was detected by Western blot.Results: Compared with sham group,the expression of miR-28-3p in the retina tissue of rats in the model group was up-regulated (P＜0.00 l).Compared with model group,the expression of miR-28-3p in the retina tissue of rats in the miR-28-3p inhibitor group was down-regulated (P＜0.001).Compared with sham group,the distribution area of blood vessels in the retina and the expression of CD31 in the model group increased significantly (both P＜0.001).Compared with model group,the distribution area of blood vessels in the retina and the expression of CD31 in the miR-28-3p inhibitor group decreased (both P＜0.01).Compared with the model group,the number of Bax cells in the miR-28-3p inhibitor group decreased (P＜0.001),but the number of Bcl-2 cells increased (P＜0.01).Compared with the sham group,the serum levels of TNF-α,IL-1β and IL-6 in the model group increased significantly (all P＜0.001),while the levels of TNF-α,IL-1β and IL-6 in the serum of the miR-28-3p inhibitor group significantly decreased (both P＜0.01).Compared with the model group,the content of PI3K and AKT protein in the retina of the miR-28-3p inhibitor group significantly decreased (both P＜ 0.01).Conclusion: miR-28-3p inhibitor can inhibit angiogenesis in the retina,reduce inflammatory response and inhibit the expression of apoptosis factor Bax,promote the expression of apoptosis inhibitor Bcl-2,and can inhibit the activation of PI3K/AKT signaling pathway,which plays an important role in the occurrence and development of DR.Therefore,miR-28-3p may be a potential target for the treatment of diabetic retinopathy.关键词
miR-28-3p/PI3K/AKT通路/糖尿病性视网膜病变/视网膜血管生成Key words
miR-28-3p/PI3K/AKT pathway/diabetic retinopathy/retinal angiogenesis分类
医药卫生引用本文复制引用
闫欢欢,李春花,曾戎,陈红,苏伟..miR-28-3p通过PI3K/AKT通路对大鼠糖尿病性视网膜病变及视网膜血管生成的影响[J].广西医科大学学报,2020,37(8):1389-1397,9.基金项目
This study was supported by Key Research& DevelopmentProjects in Shanxi Province (N0.2019SF-126). （N0.2019SF-126）
