南方医科大学学报2020，Vol.40Issue(9)：1225-1229,5.DOI:10.12122/j.issn.1673-4254.2020.09.01
血液穴位注射后小鼠的注射部位发生了什么变化?
What happened in the acupoint area of mouse after blood injection?
摘要
Abstract
Objective Autologous blood acupoint injection has practiced for treatment of various diseases, but its therapeutic mechanism remains unknown. This study aimed to explore the histological changes in the acupoint area after blood injection. Methods Blood samples (0.1 mL) collected from donator mice was injected in the recipient mice at the acupoint of left Zusanli (ST-36). At 0, 6, 12, 24, 48, 72 and 96 h after the injection (3 mice at each time point), peripheral blood and muscular tissue were collected from the injection site for blood cell classification and histological study with HE staining. Results White blood cells and lymphocytes tended to increase but platelets tended to decrease at the acupoint after blood injection, but these changes were not statistically significant. Within 24 h after blood injection, acute inflammation occurred at the acupoint, and the injected blood components were infiltrated by neutrophils;a small number of monocytes were observed at the injection site, where red blood cells gradually disappeared. From 24 to 96 h, the injected area showed chronic inflammation with infiltration by mononuclear cells, macrophages, and lymphocytes, and the number of fibroblasts increased and neutrophils decreased gradually over time;the local muscle fibers were destroyed, and proliferation of fibrous tissue could be seen. Conclusion Blood injection at the acupoints can mimic the process of hematoma absorption in the muscular tissue, and local inflammation is initiated to engulf and clear the blood components, which may be the basis for the therapeutic effects induced by acupoint stimulation.关键词
针灸/自体血液疗法/炎症/机制Key words
acupuncture/autohemotherapy/inflammation/mechanism引用本文复制引用
陈永,刘永谱,班素芬,汤瑶,邱富娟,肖长虹..血液穴位注射后小鼠的注射部位发生了什么变化?[J].南方医科大学学报,2020,40(9):1225-1229,5.基金项目
广东省中医药局科研项目(20201229) （20201229）
Supported by Scientific Research Project of Guangdong Provincial Traditional Chinese Medicine Bureau(20201229). （20201229）
