数字中医药（英文）2020，Vol.3Issue(3)：180-187,8.DOI:10.1016/j.dcmed.2020.09.004
黄精中亚油酸的定性与定量研究
Qualitative and Quantitative Analysis of Linoleic Acid in Polygonati Rhizoma
摘要
Abstract
Objective To explore the major compound in Polygonati Rhizoma (Huang Jing, 黄精) for quality control. Methods The major compound was isolated and analyzed by liquid chromatography-mass spectrometry (LC-MS), and subsequently further identified by nuclear magnetic resonance (NMR). Thin layer chromatography (TLC) was optimized based on the previous methods reported in the Chinese Pharmacopeia (2015 edition). Results The major compound was isolated from the natural material and identified as linoleic acid. A high performance liquid chromatography (HPLC) method with robust linearity (R2 = 0.999 7), specificity, precision, stability, repeatability and recovery was developed for linoleic acid determination. TLC chromatogram was improved significantly after optimization for qualitative analysis. Conclusions The optimized TLC method is practical and can be adopted for quality control of Polygonati Rhizoma (Huang Jing,黄精). The levels of linoleic acid vary between species of Polygonati Rhizoma (Huang Jing, 黄精), with Polygonatum cyrtonema Hua (Jiang Xing Huang Jing, 姜型黄精) showing the highest contents. This study provides valuable information for quality control of Polygonati Rhizoma (Huang Jing, 黄精).关键词
黄精/亚油酸/定性分析/定量分析/薄层色谱/高效液相色谱Key words
Polygonati Rhizoma(Huang Jing, 黄精)/Linoleic acid/Qualitative analysis/Quantitative analysis/Thin layer chromatography(TLC)/High performance liquid chromatography(HPLC)引用本文复制引用
周准,王炜,刘昌孝,袁汉文,彭彩云,翦雨青,周旭东,李斌,盛文兵,龚力民,何述金..黄精中亚油酸的定性与定量研究[J].数字中医药（英文）,2020,3(3):180-187,8.基金项目
We thank for the funding support from the National Standardization Construction in TCMs of China (No. ZYBZH-Y-HUN-23), National Key Research and Development Projects of China(No. 2018YFC1707903) and Key Research and Development Projects of Hunan Province (No. 2018SK2119). （No. ZYBZH-Y-HUN-23）
