首页|期刊导航|世界中医药杂志（英文版）|Ginsenoside Rb1 Pretreatment Attenuates Myocardial Ischemia by Reducing Calcium/Calmodulin-Dependent Protein Kinase Ⅱ-Medicated Calcium Release
世界中医药杂志（英文版）2020，Vol.6Issue(3)：284-294,11.DOI:10.4103/wjtcm.wjtcm_24_20
Ginsenoside Rb1 Pretreatment Attenuates Myocardial Ischemia by Reducing Calcium/Calmodulin-Dependent Protein Kinase Ⅱ-Medicated Calcium Release
Ginsenoside Rb1 Pretreatment Attenuates Myocardial Ischemia by Reducing Calcium/Calmodulin?Dependent Protein Kinase Ⅱ?Medicated Calcium Release
摘要
Abstract
Objective: The aim of this study was to investigate the protective effects of ginsenoside Rb1 and assess whether these protective effects are related to calcium/calmodulin?dependent protein kinase Ⅱ(CaMKII). Methods: A myocardial ischemia (IS) rat. model and a myocardial H9C2 cell hypoxia model were established. MI was induced by occluding the left anterior descending artery for 120?min. Ginsenoside Rb1?(10?mg/kg) was administered 30?min before ischemia induction, and the treatment continued for 7?days. Results: In the rat IS injury model, ginsenoside Rb1 reduced myocardial infarct size, mean left ventricular diastolic pressure, incidence of arrhythmia, and levels of serum creatine kinase, lactate dehydrogenase, and malondialdehyde. However, the mean left ventricular systolic pressure, and maximal rising and falling rates of ventricular pressure? (±dp/dtmax) increased. In the myocardial H9C2 cell hypoxia model, ginsenoside Rb1 reduced intracellular calcium concentrations?([Ca2+ ]i) during hypoxia, and markedly reversed the hypoxia?induced decrease in cell survival. Ginsenoside Rb1 was involved in the downregulation of CaMKⅡ and the ryanodine receptor, as well as hypoxia?induced H9C2 cell survival. Conclusion: The findings of the present study suggest that ginsenoside Rb1 attenuates MI injury in rats, partially through the downregulation of CaMKⅡ expression.关键词
Calcium/calmodulin‑dependent protein kinase Ⅱ/Ginsenoside Rb1/myocardial ischemia/ryanodine receptorKey words
Calcium/calmodulin‑dependent protein kinase Ⅱ/Ginsenoside Rb1/myocardial ischemia/ryanodine receptor引用本文复制引用
Wen‑Jun Zhou,Juan‑Li Li,Qian‑Mei Zhou,Fei‑Fei Cai,Xiao‑Le Chen,Yi‑Yu Lu,Ming Zhao,Shi‑Bing Su..Ginsenoside Rb1 Pretreatment Attenuates Myocardial Ischemia by Reducing Calcium/Calmodulin-Dependent Protein Kinase Ⅱ-Medicated Calcium Release[J].世界中医药杂志（英文版）,2020,6(3):284-294,11.基金项目
This study was supported by the National Natural Science Funds?(81073134),085 First?Class?Discipline Construction Innovation Science and Technology Support Project of Shanghai University of TCM?(085ZY1206)and E?institutes of Shanghai Municipal Education Commission?(No E 03008). （81073134）
