无机材料学报2020，Vol.35Issue(10)：1169-1176,中插7-中插9,11.DOI:10.15541/jim20200005
具有高类氧化酶活性的铂-金枝状纳米粒子用于检测抗坏血酸
Pt-Au Dendritic Nanoparticles with High Oxidase-like Activity for Detection of Ascorbic Acid
摘要
Abstract
Due to high stability and sensitivity, inorganic nanomaterials with enzyme-like activity have brilliant ap-plication prospects. Tuning the enzyme-like activity plays great significance for promoting the development of nanozymes. In this work, Pt-Au dendritic nanoparticles (Pt-Au DNPs) with good uniformity and stability were synthe-sized by a simple liquid phase reduction method, and used to colorimetrically detect ascorbic acid (AA) by using their oxidase-like activity to catalyze the oxidation of TMB (3,3′,5,5′-tetramethylbenzidine). The oxidase-like activity was found to be highly influenced by the composition and structure of Pt-Au dendritic nanoparticles, and the relationship between kinetic parameters and nanoparticle structure was investigated. Quantitative analysis of ascorbic acid in the 1–15 μmol/L range was performed with good linear relationship, and the detection limit was 78 nmol/L. At the same time, it's found that continuous reaction would reduce the catalytic performance of Pt-Au DNPs, but still has the po-tential to be reused, which is not common. Data from this research not only suggests a method for synthesizing Pt-Au DNPs, but also shows its potential for AA analysis in biological samples.关键词
Pt-Au DNPs/类氧化酶活性/比色法/检测抗坏血酸Key words
Pt-Au dendritic nanostructure/oxidase-like activity/colorimetric method/ascorbic acid分类
化学化工引用本文复制引用
程琴,杨勇,杨莉莉..具有高类氧化酶活性的铂-金枝状纳米粒子用于检测抗坏血酸[J].无机材料学报,2020,35(10):1169-1176,中插7-中插9,11.基金项目
National Key Research and Development Project(2017YFB0310600) （2017YFB0310600）
Shanghai International Science and Technology Cooperation Fund(17520711700,18520744200) （17520711700,18520744200）
