高等学校化学学报2020，Vol.41Issue(11)：2426-2434,9.DOI:10.7503/cjcu20209236
大斯托克斯位移远红光至近红外荧光探针用于检测肝损伤过程中过氧化亚硝酸盐的动态变化
Monitoring of Peroxynitrite Variation During Liver Injury Adopting a Far Red to Near-infrared Fluorescent Probe with Large Stokes Shift
摘要
Abstract
Liver injury is one of the major problems affecting public health,and has attracted growing atten-tion. It is universally believed that overexpression of peroxynitrite(ONOO-) plays an important role in the pathogenesis of liver injury and other diseases,and it is closely related to early liver injury. Therefore,in or-der to understand the role of ONOO-in the process of liver injury,an analysis method that can achieve high se-lectivity and real-time detection of ONOO-is urgently developed. Herein,we present a far red to near-infrared (FR-NIR) fluorescent probe with large Stokes shift to detect the ONOO- production in vivo during drug induced liver injury(DILI). Owing to the large Stokes shift,the FR-NIR probe(LSDQ-ONOO-)can not only reduce the interference of spectral overlap and self-absorption,but also improve the imaging signal-to-noise ratio. High sensitivity(detection limit=25.8 nmol/L)and excellent selectivity ensured that LSDQ-ONOO-can monitor subtle changes of ONOO-in vitro and in vivo during DILI.关键词
荧光探针/大斯托克斯位移/过氧化亚硝酸盐/药物诱导肝损伤Key words
Fluorescent probe/Large Stokes shift/Peroxynitrite/Drug induced liver injury分类
化学化工引用本文复制引用
吴倩,程丹,吕芸,袁林,张晓兵..大斯托克斯位移远红光至近红外荧光探针用于检测肝损伤过程中过氧化亚硝酸盐的动态变化[J].高等学校化学学报,2020,41(11):2426-2434,9.基金项目
国家自然科学基金(批准号:21877029,21735001,21904035)和中国博士后科学基金(批准号:2018M642968)资助.This paper is supported by the National Natural Science Foundation of China(Nos.21877029,21735001,21904035)and the China Postdoctoral Science Foundation(No.2018M642968). （批准号:21877029,21735001,21904035）
