水生生物学报2020，Vol.44Issue(6)：1263-1269,7.DOI:10.7541/2020.147
石斑角形虫(黏体门, 角形虫科)的分子证据及其在不同宿主中的遗传变异
FIRST MOLECULAR EVIDENCE OF CERATOMYXA EPINEPHELA (MYXOZOA:CERATOMYXIDAE) AND ITS GENETIC VARIATION FROM DIFFERENT HOST SPECIES
摘要
Abstract
Ceratomyxa epinephela Wu, Wu et Hua, 1993 is originally found to infect the gallbladder of Epi-nephelus akaara Temminck & Schlegel, 1842 from South China Sea, and no molecular data have been provided up to date. In the present study, we detected this species infecting the gallbladder of E. awoara Tem-minck&Schlegel, 1842 and E. bruneus Bloch, 1793 from East China Sea. For the first time, we offered the SSU rDNA and ITS1 rDNA information for this species and redescribed such species based on the combina-tion of morphological and molecular data. Mature spores of C. epinephela were (4.8±0.5) μm (3.6—5.6 μm) in length and (31.8±4.8) μm (23.3—37.5 μm) in thickness. Myxospores valves were smooth and almost equal, which were joined by a prominent suture. Polar capsules measured (2.9±0.2) μm (2.4—3.7 μm) in length and (2.6±0.2) μm (2.2—3.1 μm) in width. Posterior angle was slightly concave to flat, measured (175.9±3.7)°(165.5°—179.7°). Phylogenetic analysis based on SSU rDNA revealed that C. epinephela had the close relationship with C. nolani Gunter&Adlard, 2009, C. cutmorei Gunter&Adlard, 2009, and C. yokoyamai Gunter&Adlard, 2009, all of whose host were epinephelids. It also implied that the close host af-finity might have close phylogenetic relationships of their myxosporean parasites. The sequences analysis based on SSU rDNA and ITS1 rDNA revealed that the four isolates of C. epinephela had already formed dif-ferent populations, while the species did not form a specialized genetic characteristic among its different host species.关键词
石斑角形虫/重描述/SSU rDNA/ITS1 rDNA/遗传变异Key words
Ceratomyxa epinephela/Redescription/SSU rDNA/ITS1 rDNA/Genetic variation分类
生物科学引用本文复制引用
黄艳梅,赵元莙,周杨,杨承忠..石斑角形虫(黏体门, 角形虫科)的分子证据及其在不同宿主中的遗传变异[J].水生生物学报,2020,44(6):1263-1269,7.基金项目
Supported by the National Natural Science Foundation of China (31501845, 31672280, 31970409) （31501845, 31672280, 31970409）
Chongqing Natural Sci-ence Foundation (cstc2017jcyjAX0165 and cstc2018jcyjAX0738) （cstc2017jcyjAX0165 and cstc2018jcyjAX0738）
Venture & Innovation Support Program for Chongqing Overseas Returnees (cx2018108) （cx2018108）
Science and Technology Research Program of Chongqing Municipal Education Commis-sion (KJQN201900505) （KJQN201900505）
