广西医科大学学报2020，Vol.37Issue(11)：1931-1941,11.DOI:10.16190/j.cnki.45-1211/r.2020.11.001
miR-451通过靶向ROCK1对卵巢癌细胞侵袭和迁移能力的调控作用
Regulation of miR-451 on invasion and migration of ovarian cancer cells by targeting ROCK1
摘要
Abstract
Objective: To investigate the effect and mechanism of miR-451 on invasion and migration of ovarian cancer cells.Methods: The expressions of miR-451 and ROCK1 mRNA in ovarian cancer tissues and cells,paracancerous tissues and normal ovarian epithelialcells were detected by RT-qPCR.Ovarian cancer CaOV-3 cells were divided into 6 groups: Control group (non-transfection group),miR-NC group (transfected with miR-NC disordered sequence),miR-451 mimic group (transfected with over-expressed miR-451 mimics),miR-451 inhibitor group (transfected with plasmids inhibiting miR-451 expression),pc-ROCK1 group (transfected with plasmids overexpressing ROCK1) and miR-451 + pcROCK1 group (co-transfected with miR-451 mimics and plasmids overexpressing ROCK1).The ability of cell migration was detected by scratch test.The invasive ability of cells wasdetected by Transwell method.The protein expression levels of E-cadherin,N-cadherin,MMP2 and ROCK1 were detected by Western blot.The target gene of miR-451 was predicted by TargetScan database.The targeting relationship between miR-451 and ROCK1 was detected by double luciferase report.Results:Compared with paracancerous tissues,miR-451 expression was down-regulated in most ovarian tissues (57/70,81.43％).Patients with low expression of miR-451 had larger ovarian cancer tumors,poorer differentiation,mostly belonged to the late stage of FIGO,and had obvious peritoneal metastasis (P＜0.05).Compared with the Control group,the scratch closure rate of ovarian cancer CaOV-3 cells in the miR-451 mimic group was significantly reduced,the number of invading cells was significantly reduced,E-cadherin was significantly up-regulated,and N-cadherin and MMP2 expression were significantly down-regulated (P＜0.01).Compared with the Control group,the scratch closure rate of ovarian cancer CaOV-3 cells in miR-451 inhibitor group was significantly increased,the number of invading cells was significantly increased,E-cadherin was significantly down-regulated,and N-cadherin and MMP2 expression were significantly up-regulated (P＜0.01).miR-451 down-regulated ROCK1.Co-transfection of pcROCK1 reversed the effect of miR-451 mimic on the invasion and migration of ovarian cancer CaOV-3 cells(P＜0.0l).Conclusion:MiR-451 inhibits the migration and invasion of ovarian cancer CaOV-3 cells by targeting down-regulation ofRCOK1.关键词
miR-451/RCOK1/卵巢癌/迁移/侵袭Key words
miR-451/RCOK1/ovarian cancer/migration/invasion分类
医药卫生引用本文复制引用
黄丽,龚豪,张春莲,方彩云,包媛媛..miR-451通过靶向ROCK1对卵巢癌细胞侵袭和迁移能力的调控作用[J].广西医科大学学报,2020,37(11):1931-1941,11.基金项目
This study was funded by Natural Science Foundation of Hubei Province (No.2017CFB482). （No.2017CFB482）
