数字中医药（英文）2020，Vol.3Issue(4)：275-282,8.DOI:10.1016/j.dcmed.2020.12.006
家蚕提取物对2型糖尿病大鼠降糖作用及胰岛保护作用的研究
Silkworm Extract Ameliorates Type 2 Diabetes Mellitus and Protects Pancreatic β-cell Functions in Rats
摘要
Abstract
Objective The aim of the present study was to investigate the hypoglycemic effects of silkworm extract (SE) on experimental type 2 diabetes mellitus (T2DM) rats.Methods SE was prepared by dissolving freeze-dried silkworm powder in 70％ (v/v) aqueous ethanol. T2DM rats were induced by feeding them a high fat diet and an intraperitoneal injection of streptozotocin (STZ). The blood glucose, free fatty acid (FFA), malondialdehyde (MDA), tumor necrosis factor alpha (TNF-α) and superoxide dismutase (SOD) levels were detected by enzyme-linked immunosorbent assay (ELISA). The quality of SE was controlled by high performance liquid chromatography (HPLC; Agilent 1260, Agilent, USA). Hematoxylin-eosin (HE) staining was performed for histological evaluation. Antibody expression was assessed via immunohistochemistry (IHC) staining. Results SE could improve insulin resistance and islet cell function by reducing FFA, MDA and TNF-α levels and increasing SOD level. In addition, pancreatic HE staining analysis revealed that SE has a protective effect on islet β-cells. Conclusions The present study indicates that SE has hypoglycemic as well as pancreatic protective effects in T2DM model rats.关键词
家蚕提取物/降血糖活性/2型糖尿病/胰岛素抵抗/胰岛保护Key words
Silkworm extract/Hypoglycemic activity/Type 2 diabetes mellitus (T2DM)/Insulin resistance/Pancreatic protection引用本文复制引用
万慧琪,戚欢阳,许光辉,黄亦琦..家蚕提取物对2型糖尿病大鼠降糖作用及胰岛保护作用的研究[J].数字中医药（英文）,2020,3(4):275-282,8.基金项目
This study was supported by the Xiamen City Health and Family Planning Commission and the Xiamen Municipal Bureau of Science and Technology.We thank for the funding support from the Leading Project of Fujian.P.R.C.(No.2019D018)and the Xiamen Science and Technology Project(No.3502Z20174058). （No.2019D018）
