数字中医药（英文）2020，Vol.3Issue(4)：297-308,12.DOI:10.1016/j.dcmed.2020.12.008
益气解毒方联合盐霉素通过CD44/Ras信号通路调控鼻咽癌干细胞增殖、迁移和凋亡
Yi Qi Jie Du Formula and Salinomycin Combination Treatment Mediates Nasopharyngeal Carcinoma Stem Cell Proliferation, Migration and Apoptosis via CD44/Ras Signaling Pathway
摘要
Abstract
Objective To assess the effects of Yi Qi Jie Du Formula (YQJDF) combined with salinomycin (SAL) on nasopharyngeal carcinoma stem cells (NPC-SCs) and investigate the underlying molecular mechanisms. Methods Cell counting methods, the CCK-8 assay, transwell migration assay and JC-1 staining, were used to observe the effects of the combination on the proliferation, migration and apoptosis of NPC stem cells, respectively. Western blot was used to detect the levels of protein in NPC-SCs. Results YQJDF combined with SAL had a synergistic effect on the inhibition of proliferation and migration and induction of NPC-SC apoptosis. Mechanistically, YQJDF combined with SAL synergistically upregulated the levels of apoptotic proteins, including cleaved Caspase-3, cleaved Caspase-7 and cleaved Caspase-9. Moreover, YQJDF combined with SAL synergistically decreased the levels of CD44, p-c-Src, Ras, p-PKCδ, p-MEK, p-c-Raf, p-ERK1/2 and p-AKT proteins. Conclusions The combination of YQJDF and SAL has a synergistic effect on the inhibition of NPC-SC proliferation and migration and induction of apoptosis, which may be closely related to the downregulation of the CD44/Ras signaling pathway.关键词
益气解毒方/盐霉素/鼻咽癌干细胞/增殖/凋亡/CD44/Ras信号通路Key words
Yi Qi Jie Du Formula (YQJDF)/Salinomycin/Nasopharyngeal carcinoma stem cells (NPC-SCs)/Proliferation/Apoptosis/CD44/Ras signaling pathway引用本文复制引用
何兰,周芳亮,邹攀,王贤文,蒋益兰,何迎春,廖端芳,CAO De-Liang..益气解毒方联合盐霉素通过CD44/Ras信号通路调控鼻咽癌干细胞增殖、迁移和凋亡[J].数字中医药（英文）,2020,3(4):297-308,12.基金项目
We thank for the funding support from the National Natural Science Foundation of China(No.81874408,No.81973914 and No.81573721),the Natural Science Foundation of Hunan Province,China(No.2019JJ40216)and Study Fundation of Hunan Provincial Education Department(No.19B439). （No.81874408,No.81973914 and No.81573721）
