广西医科大学学报2020，Vol.37Issue(12)：2095-2102,8.DOI:10.16190/j.cnki.45-1211/r.2020.12.001
LncRNA BCAR4调控JAK2/STAT3信号通路对食管癌细胞侵袭、迁移、炎症和凋亡的影响
LncRNA BCAR4 affects the migration,invasion,inflammation and apoptosis of esophageal cancer cells through regulation of JAK2/STAT3 pathway
摘要
Abstract
Objective:To investigate the effect of long-chain non-coding RNA(lncRNA)breast cancer anti-estrogen resis-tance 4(BCAR4)-mediated JAK kinase 2/signal transduction and transcription factor 3(JAK2/STAT3)signal pathway on mi-gration,invasion,apoptosis and inflammation of esophageal cancer cells.Methods:The expression of BCAR4 in esophageal cancer cells was detected by qPCR.BCAR4 small interference RNA(si-BCAR4)was transfected in esophageal cancer EC9706 cells.Cell migration,invasion and apoptosis were de-tected by Transwell and flow cytometry.The expressions of in-terleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),interleu-kin-6(IL-6),phosphorylated JAK2(pJAK2),JAK2,phosphor-ylated STAT3(pSTAT3)and STAT3 were detected by Western blot.EC9706 cells were treated with Colivelin,an activator of JAK2/STAT3 signal pathway,and to observe the role of EC9706 cells in migration,invasion,apoptosis and expression of inflammatory factors induced by BCAR4 silencing in esoph-ageal cancer cells.Results:Compared with Het-1A cells of nor-mal esophageal squamous epithelium,the expression of BCAR4 in esophageal cancer cells TE-10,OE19 and EC9706 was significantly increased(P<0.05).BCAR4 silencing signif-icantly decreased the number of migration and invasion,the ex-pression of IL-1β,TNF-α,IL-6,pJAK2 and pSTAT3(P<0.05),and increased the apoptosis rate of EC9706 cells(P<0.05),but had no significant effect on the protein levels of JAK2 and STAT3(P>0.05).Activation of JAK2/STAT3 pathway re-versed the inhibition of BCAR4 silence on migration,invasion,and expression of inflammatory factors of EC9706 cell,and the induction of apoptosis.Conclusion:LncRNA BCAR4 si-lencing can inhibit the migration,invasion and secretion of in-flammatory factors and promote apoptosis of esophageal can-cer cells by regulating the activity of JAK2/STAT3 signal path-way.关键词
BCAR4/JAK2/STAT3信号通路/食管癌/迁移/侵袭/凋亡/炎症Key words
BCAR4/JAK2/STAT3 signal pathway/esophageal cancer/migration/invasion/apoptosis/inflammation分类
医药卫生引用本文复制引用
彭文,范长玲,张浩..LncRNA BCAR4调控JAK2/STAT3信号通路对食管癌细胞侵袭、迁移、炎症和凋亡的影响[J].广西医科大学学报,2020,37(12):2095-2102,8.基金项目
This study was funded by Health Commission of Hainan Province(No.1605320273A-2001). （No.1605320273A-2001）
