广西医科大学学报2020，Vol.37Issue(12)：2103-2110,8.DOI:10.16190/j.cnki.45-1211/r.2020.12.002
岩黄连总碱对巨噬细胞M1型分化的抑制作用
Inhibitory effects of Corydalis saxicola Bunting total alkaloid on M1 macrophages polarization
摘要
Abstract
Objective:To investigate the effects of Corydalis saxicola Bunting total alkaloid(CSBTA)on classically activat-ed type 1(M1)polarization of macrophages(Mψ).Methods:THP-1 cells were induced to differentiate into Mψ by phorbol ester(PMA)for 48 h.Morphological change of the cells was observed using inverted microscope.Flow cytometry was ap-plied to detect the myloid cell markers CD11b and CD14,as well as the major marker of Mψ.CSBTA was given to M1-Mψwhich was induced from M0-Mψ by interferon(IFN-γ,20 ng/mL)and lipopolysaccharide(LPS,1 μg/mL),at various con-centrations ranging from 0.002 5 g/L to 0.02 g/L for 24 h.The mRNA levels of tumor necrosis factor(TNF)-α and interleukin(IL)-6 were detected by quantitative real-time PCR(RT-qP-CR).The expression level of CD86 was determined by western blotting.The amount of IL-1β in the cell culture supernatant was measured by enzyme linked immunosorbent assay(ELI-SA).Results:0.02 g/L of CSBTA had a significant toxic effect on the vitality of M1-Mψ(P<0.05).0.002 5 g/L and 0.005 g/L of CSBTA significantly reduced the mRNA levels of IL-6 and TNF-α(P<0.05),the protein expression of CD86 in M1-Mψand the production of IL-1/β in the cell culture supernatant(P<0.05).Conclusion:CSBTA can effectively suppress M1 polar-ization of THP-1-derived Mψ that may improve the inflamma-tory environment.关键词
岩黄连总碱/巨噬细胞/分化Key words
Corydalis saxicola Bunting total alkaloid/macro-phages/polarization分类
医药卫生引用本文复制引用
冯葱,雍翔智,江巧芝,吴恬恬,蒋兰岚,苏志恒,陶人川..岩黄连总碱对巨噬细胞M1型分化的抑制作用[J].广西医科大学学报,2020,37(12):2103-2110,8.基金项目
This research was supported by grants from the National Natural Science Foundation of China(81771073) （81771073）
Innovation Project of Guangxi Graduate Education(YCSW2020127) （YCSW2020127）
Youth Science Foundation of Guangxi Medical University(GX-MUYSF201813). （GX-MUYSF201813）
