中南民族大学学报（自然科学版）2021，Vol.40Issue(1)：43-49,7.DOI:10.12130/znmdzk.20210108
葡萄籽中原花青素定量方法的比较(英文)
Comparison of different methods for quantification of proanthocyanidins from grape seeds
摘要
Abstract
The effect of extraction temperature,time,solvent and the number of cycles on the solid-liquid extraction yield of total polyphenols and three main phenolic compounds in grape seed extracts were investigated. The optimal extraction conditions were obtained as follows:Extraction temperature 50 ℃ and 60 min of extraction with 80 : 20 ( ethanol/water, V/V) of aqueous ethanol for four successive extractions.Additionally,quantification of proanthocyanidins from grape seeds by vanillin-HCl assay and Folin-Ciocalteu assay in combination with HPLC was evaluated,and compared with acid butanol assay.The results showed that vanillin-HCl-HPLC method and acid butanol assay were more efficient than Folin-Ciocalteu-HPLC method for the quantification of proanthocyanidin,and there was no significant difference( P>0.05) between vanillin-HCl-HPLC and acid butanol assay.Especially,acid butanol assay provides a simple,fast and effective method to quantify the proanthocyanidin,and could be a better alternative to vanillin-HCl-HPLC method and Folin-Ciocalteu-HPLC method of analyzing the proanthocyanidin in grape seed extracts or other natural sources.关键词
葡萄籽/原花青素/定量/芳草醛-HCl测定法/Folin-Ciocalteu测定法/酸性丁醇测定法Key words
grape seed/proanthocyanidin/quantification/Vanillin-HCl assay/Folin-Ciocalteu method/acid butanol assay分类
化学化工引用本文复制引用
周莉,杨富,张明浩,姚家旭..葡萄籽中原花青素定量方法的比较(英文)[J].中南民族大学学报（自然科学版）,2021,40(1):43-49,7.基金项目
国家自然科学基金资助项目(31501521) （31501521）
中央高校基本科研业务费专项资金资助项目(CZY19032) （CZY19032）
