分析测试学报2021，Vol.40Issue(1)：27-35,9.DOI:10.3969/j.issn.1004-4957.2021.01.004
低温制备高纯度番茄红素异构体及其在食用油中的异构化及降解研究
Study on Low-temperature Preparation of High-purity Lycopene Isomers and Their Isomerization and Degradation in Edible Oils
摘要
Abstract
In this work,an orthogonal separation method was developed for the separation of lyco-pene isomers based on the combination of low temperature(L-T)C18 column and C30 column.As the temperature decreased to-20℃,the resolution for the separation of lycopene isomers on a nor-mal C18 column was greatly improved.After investigating the relation of solubility and resolution,seven kinds of lycopene isomers were finally prepared by the orthogonal separation method with their purities all higher than 97％.The loading capacity and purification effect of the orthogonal method for lycopene were much higher than that of the method only based on L-T C18 or C30 column.Then,the obtained all-trans lycopene were dissolved in walnuts oil,black seed oil,almonds oil,grape seed oil,pumpkin seed oil and peach kernel oil,respectively,and heated at 120 ℃ to study their isom-erization.Grape seed oil was regarded as the most valuable solvent for developing lycopene product due to its highest cis/all-trans ratio of lycopene isomers after heating.Finally,the stability of cis-iso-mers at 4 ℃ in edible oils was investigated by taking walnut oil as the example.Both the isomeriza-tion and degradation of these isomers happened with the increase of storage time.All-trans lycopene and(13Z)lycopene were found as the preferential conformations in walnut oil.The obtained results were expected to provide some beneficial information for the development of lycopene product.关键词
番茄红素/低温制备/异构化/降解/食用油Key words
lycopene/low temperature preparation/isomerization/degradation/edible oils分类
化学化工引用本文复制引用
迪娜·波拉提,孙光映,侯雪玲,阿吉艾克拜尔·艾萨..低温制备高纯度番茄红素异构体及其在食用油中的异构化及降解研究[J].分析测试学报,2021,40(1):27-35,9.基金项目
The"West Light"Talent Training Plan of the Chinese Academy of Sciences(2017-XBQNXZ-B-007) （2017-XBQNXZ-B-007）
Special research items of Chinese Academy of Sciences(E0290703) （E0290703）
