广西医科大学学报2021，Vol.38Issue(1)：1-8,8.DOI:10.16190/j.cnki.45-1211/r.2021.01.001
miR-200a-3p靶向转甲状腺素蛋白对妊娠期高血压疾病滋养细胞生物学行为的影响
Effect of miR-200a-3p targeting transthyretin(TTR)on the biological behavior of placental trophoblasts in hypertensive disorder complicating pregnancy
摘要
Abstract
Objective:To investigate the expression of mi-croRNA-200a-3p(miR-200a-3p)in placental tissues of pa-tients with hypertensive disorder complicating pregnancy(HD-CP)and its effect on the biological behavior of trophoblasts.Methods:Fifty cases of HDCP(study group)and 50 normal pregnant women(control grouo)in our hospital from January 2017 to May 2019 were collected.The expressions of miR-200a-3p and transthyretin(TTR)mRNA in placental tissues were detected by fluorescent quantitative PCR(qPCR).MiR-200a-3p mimics,inhibitor and negative control were transfect-ed into human chorionic carcinoma JEG-3 cells by liposome.Cell proliferation,migration,invasion and apoptosis were de-tected by CCK-8 method,Transwell test and Annexin V-FITC/PI test,respectively.Bioinformatics and dual luciferase reporter gene experiments were used to analyze the targeting relation-ship between miR-200a-3p.Western blottingwas used to detect the TTR expression.Results:The expression level of miR-200a-3p in study group was significantly higher than that incontrol group,while the expression level of TTR mRNA in study group was significantly lower than that incontrol group(P＜0.01).Transfection of miR-200a-3p mimic to overexpress miR-200a-3p in could significantly promote the proliferation,inva-sion and migration of JEG-3 cells,and inhibit apoptosis(P＜0.01).Transfection of miR-200a-3p inhibitor to inhibit the ex-pression of miR-200a-3p could significantly inhibit the prolif-eration,invasion and migration of JEG-3 cells,and promote apoptosis(P＜0.01).MiR-200a-3p could significantly decrea-se the luciferase activity of TTR-WT(P＜0.01).Transfection of miR-200a-3p mimic could significantly decreased the expres-sion of TTR protein,while the result of transfection of miR-200a-3p inhibitor was on the contrary(P＜0.01).Conclusion:miR-200a-3p is highly expressed in placental tissue of HDCP patients.Inhibition of miR-200a-3p expression in JEG-3 cells can inhibit cell proliferation,migration,invasion,and promote apoptosis.MiR-200a-3p may participate in the pathological mechanism of HDCP through negative regulation of TTR.关键词
微小RNA-200a-3p/转甲状腺素蛋白/妊娠期高血压疾病/JEG-3细胞/生物学行为Key words
microRNA-200a-3p/transthyretin/hypertensive disorders complicating pregnancy/JEG-3 cells/biological be-havior分类
医药卫生引用本文复制引用
刘莹,刘海娟,黄志娟..miR-200a-3p靶向转甲状腺素蛋白对妊娠期高血压疾病滋养细胞生物学行为的影响[J].广西医科大学学报,2021,38(1):1-8,8.基金项目
This study was funded by Key Research and Development Project of Shanxi Prov-ince(No.2019SF-127). 基金项目:陕西省重点研发计划项目(No.2019SF-127) （No.2019SF-127）
