广西医科大学学报2021，Vol.38Issue(2)：217-228,12.DOI:10.16190/j.cnki.45-1211/r.2021.02.001
与脐带间充质干细胞共培养对脂多糖诱导的牙髓细胞炎症的影响
Effect of co-culture with umbilical cord mesenchymal stem cells on lipopolysaccharide-induced inflammation in dental pulp cells
摘要
Abstract
Objective:To investigate the regulation effect of the co-culture of human dental pulp cells(hDPCs)and human umbilical cord mesenchymal stem cells(hUCMSCs)onli-popolysaccharide(LPS)-induced inflammatory microenviron-ment.Methods:To get suitable concentration of LPS for simu-lating inflammatory responses,hDPCs and hUCMSCs were cultured with different concentrations of LPS(1,10,20 and 50μg/mL).Then,direct and indirect co-culture system of hUC-MSCs and hDPCs at ratio of 1:1 were established in vitro with LPS of 10 μg/mL.Cell proliferation was detected by MTT assay.The mRNA and protein expression levels of inter-leukin(IL)-6,IL-10 and hepatocyte growth factor(HGF)were analyzed by quantitative reverse-transcription polymerase chain reaction(qRT-PCR)and enzyme-linked immunosorbent assay(ELISA),respectively.Results:LPS of 10 μg/mL could induce cell inflammation.The direct co-culture system en-hanced cell proliferation,increased mRNA and protein levels of IL-10 and HGF,while decreased the level of IL-6 compared with hDPCsmonocultured with LPS(P＜0.05).In indirect co-culture system,the protein levels of IL-6,IL-10 and HGF showed the same trend.Additionally,hDPCs from the co-cul-ture system expressed extremely higher mRNA levels of IL-10 and HGF,but lower level of IL-6 compared with hDPCs mono-cultured with LPS(P＜0.05.Conclusion:Co-culture with hUCMSCs might attenuate LPS-induced dental pulp cell in-flammation within a certain range in vitro.关键词
脐带间充质干细胞/牙髓细胞/共培养/脂多糖/炎症因子Key words
umbilical cord mesenchymal stem cells/dental pulp cells/co-culture/lipopolysaccharide/cytokines分类
医药卫生引用本文复制引用
何凯丽,刘玉莹,林田,陆彦玲,吴煜..与脐带间充质干细胞共培养对脂多糖诱导的牙髓细胞炎症的影响[J].广西医科大学学报,2021,38(2):217-228,12.基金项目
This study was supported by the National Natural Science Foundation of China(No.81660182 （）
No.82060200). （）
