解放军医药杂志2021，Vol.33Issue(3)：22-29,8.DOI:10.3969/j.issn.2095-140X.2021.03.005
RCCS模拟微重力环境对人胃黏膜上皮GES-1细胞代谢组学的影响
Effects of Simulated Microgravity Environment by RCCS on Metabolomics of GES-1 Cells in Human Gastric Mucosa
摘要
Abstract
Objective To investigate the effects of simulated microgravity (SMG) environment by rotary cell culture system (RCCS) on metabonomics of human gastric epithelial GES-1 cells. Methods Human gastric epithelial GES-1 cells were cultured in vitro. Three to ten generations of cells in logarithmic growth phase were selected and randomly divided into SMG group and normal gravity group (NG group. Cells in different groups were cultured for 1，3，and 5 d，and then subjected to non-targeted LC/MS based metabolomics analysis. Orthogonal partial least squares discriminant analysis (OPLS-DA) and two sample independent t-test were used to find the differential metabolites of the two groups of cells，and then the differential metabolites were input into KEGG database to construct metabolic pathways and conduct functional analysis. Results GES-1 cells in both groups had 151 differential metabolites after 1 d of RCCs culture，113 of which were up-regulated and 38 down-regulated;134 of which were up-regulated and 25 down-regulated after 3d of RCCS culture; 154 of which were up-regulated and 23 down-regulated after 5 d of RCCS culture. Seventy-four kinds of common differential metabolites (variable importance>1 and P<0.05) at 1，3 and 5 <1 in two groups were screened out by Venn diagram, among which 57 were up-regulated and 17 were down-regulated. KEGG database analysis found that there were 44 differential metabolites with specific names and related functions，mainly including phospholipids，amino acids，cofactors and carboxylic acids，mainly involving lipid metabolism, amino acid metabolism, coenzyme and vitamin metabolism, neurotransmitter metabolism，carbohydrate metabolism, cell proliferation and apoptosis，tumor regulation，membrane transport and signal transduction pathways. Conclusion SMG environment by RCCS can significantly affect the metabolism of GES-1 cells，which mainly involves lipid metabolism, membrane structure damage, cell proliferation and apoptosis，and tumor regulation.关键词
失重模拟/旋转式细胞培养系统/胃黏膜上皮/GES-1细胞/代谢组学Key words
Weightlessness simulation/Rotary cell culture system/ Gastric mucosal epithelium/ GES-1 cells/ Metabolomics分类
医药卫生引用本文复制引用
郭松,陈正阳,杨佳启,柴韶斌,徐冰心,孙宏伟,周金莲,杨鹤鸣,崔彦..RCCS模拟微重力环境对人胃黏膜上皮GES-1细胞代谢组学的影响[J].解放军医药杂志,2021,33(3):22-29,8.基金项目
全军试验技术研究计划重点项目(SYFD1500128) （SYFD1500128）
国家重大基础科学研究计划项目(2013CB945501) （2013CB945501）
