花生学报2021，Vol.50Issue(1)：1-11,11.DOI:10.14001/j.issn.1002-4093.2021.01.001
茉莉酸和乙烯信号转导途径参与花生应答果腐病病原菌侵染的转录组测序分析
Transcriptomic Profiling Reveals the JA and ET Signal Transduction during Field Peanut Pod Rot Pathogen Infection
摘要
Abstract
Pod rot, an important disease affecting peanut production, causes serious damage to pea-nut yield and quality.In this study, RNA sequencing analysis was performed with peanut cultivar Huayu20 kernels that infected with pod rot disease in field, and that uninfected kernels acting as control. As a result, a total of 60,756 unigenes were obtained, among which the expression of 10,147 genes were up-regulated and 4,334 genes were down-regulated.Further, Gene Ontology enrichment analysis showed that the differentially expressed genes (DEGs) were assigned to 24 cellular components, 5 molecular func-tions and 23 biological processes.Through Kyoto Encyclopedia of Genes and Genomes enrichment analy-sis, a total of 4,291 DEGs were mapped to 34 metabolic pathways.We identified multiple hormone sig-nal transduction pathways that were responsive to pod rot disease infection.Based on the expression pattern of related genes, we speculate that peanuts may resist pod rot disease infection through jas-monic acid-and ethylene-mediated pathways.关键词
花生/果腐病/转录组测序/JA/ET信号转导途径Key words
peanut/pod rot disease/transcriptome/JA and ET signal transduction pathway分类
农业科技引用本文复制引用
王冕,郭鑫钢,迟晓元,王通,陈明娜,杨珍,禹山林,陈娜,许静,潘丽娟,张朝昕..茉莉酸和乙烯信号转导途径参与花生应答果腐病病原菌侵染的转录组测序分析[J].花生学报,2021,50(1):1-11,11.基金项目
国家自然科学基金项目(31701464) （31701464）
国家花生产业技术体系项目(CARS-13) （CARS-13）
泰山学者工程专项(tsnq201812121) （tsnq201812121）
山东省自然科学基金项目(ZR2020MC103) （ZR2020MC103）
山东省农业科学院农业科技创新工程(CXGC2016B02,CXGC2018E21) （CXGC2016B02,CXGC2018E21）
山东省重大科技创新工程项目(2019JZZY010702) （2019JZZY010702）
广东省重点领域研发计划项目(2020B020219003) （2020B020219003）
青岛市科技惠民示范引导专项(20-3-4-26-nsh) （20-3-4-26-nsh）
临沂市重点研发计划(2019YD009) （2019YD009）
