皮肤性病诊疗学杂志2021，Vol.28Issue(1)：1-6,6.DOI:10.3969/j.issn.1674-8468.2021.01.001
红色毛癣菌、石膏样小孢子菌对人角质形成细胞TLR2/c-Jun、NF-κB信号通路的差异性诱导
Differential induction of TLR2/c-Jun and NF-κB signaling pathway in human keratinocytes infected by Trichophyton rubrum and Micros-porum gypseum
摘要
Abstract
Objective:To explore the activation of TLR2/c-Jun and NF-κB signaling pathway in hu-man keratinocytes infected by different ecological classifications of dermatophytes.Methods:Total and phosphorylated p65 ,relb,IκB,and c-Jun in HaCaT cells were detected by immunoblotting following co-culture with either Trichophyton rubrum (T.rubrum ) or Microsporum gypseum (M.gypseum ) for 24 h.Immunoblotting was used to assess expression levels of TLR2,c-Jun,and phosphorylated c-Jun after co-culture of TLR2 deficient HaCaT cells (HaCaT TLR2 -/-cells)with T.rubrum and M.gypse-um for 24 h.Results:Co-culture of HaCaT cells with M.gypseum,but not T.rubrum,significantly in-creased the levels of phosphorylated c-Jun.However,neither T.rubrum nor M.gypseum induced signif-icant changes in the total and phosphorylated p56,relb,and IκB in HaCaT cells.Following TLR2 knockout in HaCaT cells,the level of phosphorylated c-Jun decreased significantly in HaCaT TLR2 -/-cells co-cultured with T.rubrum,but not M.gypseum.Conclusions:T.rubrum and M.gypseum can activate c-Jun signaling pathway through TLR2 in keratinocytes,without activation of NF-κB signaling pathway.TLR2,together with other receptors,may dominate the differential induction of c-Jun pathway in keratinocytes infected by T.rubrum and M.gypseum.关键词
角质形成细胞/红色毛癣菌/石膏样小孢子菌/TLR2/c-Jun信号通路Key words
keratinocytes/Trichophyton rubrum/Microsporum gypseum/TLR2/c-Jun signa-ling pathway分类
医药卫生引用本文复制引用
苏真,邓威威,湛舒婷,梁盼盼,李美容,尹颂超,陈剑..红色毛癣菌、石膏样小孢子菌对人角质形成细胞TLR2/c-Jun、NF-κB信号通路的差异性诱导[J].皮肤性病诊疗学杂志,2021,28(1):1-6,6.基金项目
国家自然科学基金(编号:81573058) （编号:81573058）
