世界中医药杂志（英文版）2021，Vol.7Issue(1)：6-10,5.DOI:10.4103/wjtcm.wjtcm_28_20
Simultaneous Quantitative Analysis of Five Components in Angelica sinensis and Angelica acutiloba Acclimatized Growing in Vietnam by High-Performance Liquid Chromatography with Photodiode Array Detector
Simultaneous Quantitative Analysis of Five Components in Angelica sinensis and Angelica acutiloba Acclimatized Growing in Vietnam by High?Performance Liquid Chromatography with Photodiode Array Detector
Thi Minh Tam Pham 1Thi Thu Cuc Le 1Van Thanh Nguyen 2Viet Hung Tran2
作者信息
- 1. Departments of Microbiology,Institute of Drug Quality Control,Ho Chi Minh City
- 2. Departments of Instrumental Analysis,Institute of Drug Quality Control,Ho Chi Minh City
- 折叠
摘要
Abstract
Background: Chinese Danggui (Angelica sinensis) and Japanese Danggui (Angelica acutiloba) are evaluated as the same in using and critical of quality control in Vietnamese Pharmacopoeia. In Vietnam, Japanese Danggui were acclimatized and cultivated in several regions in recent years. Despite the huge climatic difference between Vietnam and Japan, Japanese Danggui grows very well in Vietnam. However, there are no studies to assess the overall quality of this medicinal herbs. Aims and Objectives: The aim of this study is to develop a method to identify and assay simultaneously 5 component compounds of these crude herbs: chlorogenic acid, ferulic acid, scopoletin, xanthotoxin and ligustilide by high-performance liquid chromatography with photodiode array detector (HPLC-PDA). Materials and Methods: The procedure was optimized by using column Phenomenex Gemini 5 μm PR C18, 15 x 4,6 mm with the detection wavelength set at 321 nm for detector PDA and mobile phase was composed of (A) ACN and (B) aqueous solution 1％ of acid acetic using a gradient elution. Analytes were performed at 30℃ with a flow rate of 1.0 mL/min. Results: The developped method were fully validated for linearity, accuracy, recovery and met the validation requirements, proved practical applicability for the quality control of these herbs and related products. Conclusion: The method was successfully applied to the quantification of five markers simultaneously from collected samples.关键词
Chlorogenic acid/Danggui/ferulic acid/ligustilide/scopoletin/xanthotoxinKey words
Chlorogenic acid/Danggui/ferulic acid/ligustilide/scopoletin/xanthotoxin引用本文复制引用
Thi Minh Tam Pham,Thi Thu Cuc Le,Van Thanh Nguyen,Viet Hung Tran..Simultaneous Quantitative Analysis of Five Components in Angelica sinensis and Angelica acutiloba Acclimatized Growing in Vietnam by High-Performance Liquid Chromatography with Photodiode Array Detector[J].世界中医药杂志（英文版）,2021,7(1):6-10,5.
