首页|期刊导航|世界中医药杂志（英文版）|Comprehensive Metabolic Profiling of Modified Gegen Qinlian Decoction by Ultra-high-Performance Liquid Chromatography-Diode Array Detection-Q-Exactive-Orbitrap-Electrospray Ionization-Mass Spectrometry/Mass Spectrometry
Comprehensive Metabolic Profiling of Modified Gegen Qinlian Decoction by Ultra-high-Performance Liquid Chromatography-Diode Array Detection-Q-Exactive-Orbitrap-Electrospray Ionization-Mass Spectrometry/Mass Spectrometry
Xuehong Nöst Eva‑Maria Pferschy‑Wenzig Xiao‑Tong Yu Min Li Xiao‑Lin Tong Rudolf Bauer
世界中医药杂志（英文版）2021，Vol.7Issue(1)：11-32,22.
世界中医药杂志（英文版）2021，Vol.7Issue(1)：11-32,22.DOI:10.4103/wjtcm.wjtcm_63_20
Comprehensive Metabolic Profiling of Modified Gegen Qinlian Decoction by Ultra-high-Performance Liquid Chromatography-Diode Array Detection-Q-Exactive-Orbitrap-Electrospray Ionization-Mass Spectrometry/Mass Spectrometry
摘要
Abstract
Objective: Gegen Qinlian decoction (GQD) is a classical traditional Chinese medicine formulation which has been used for almost 2000 years. At Guang'anmen Hospital, Beijing, a modified GQD version (mGQD) with seven instead of four herbal ingredients has been applied to treat Type 2 diabetes. Quality control is a crucial prerequisite for the therapeutic application of herbal medicines. For the identification of products derived from classical GQD, the Chinese Pharmacopeia requires the analysis of only three marker compounds. Because mGQD is a more complex mixture containing seven herbs and hundreds of constituents, the pharmacopoeia method for GQD is inadequate. Materials and Methods: A more comprehensive characterization of the formula's constituents has been developed using ultra?high?performance liquid chromatography?diode array detection (UHPLC?DAD)?Q?Exactive?mass spectrometry (MS) in electrospray ionization positive and negative mode. Moreover, a new method for the fingerprint analysis of mGQD via high-performance thin-layer chromatography (HPTLC) has been established. Results: Altogether, 91 compounds have been assigned to their originating plants and 84 substances were identified either by comparison with authentic references or with data from the literature. The HPTLC method is based on the application of two different mobile phases and is able to detect both lipophilic and hydrophilic constituents of mGQD. Conclusions: The modified GQD was extensively characterized by UHPLC combined with DAD and Q?Exactive Orbitrap high?resolution MS detection, leading to the assignment and identification of compounds present in the decoction. In addition, a new method for the fingerprint analysis of the mGQD using HPTLC was established, which allows fast and simple identification of the herbal ingredients in the mixture.关键词
Fructus jujubae/HPTLC/Modified gegen qinlian decoction/Radix et rhizoma glycyrrhizae praeparata cum melle/Radixpuerariae lobatae/Radix scutellariae/Rhizoma coptidis/Rhizoma anemarrhenae/Rhizoma zingiberis/UHPLC-DAD-Q-Exactive-MSKey words
Fructus jujubae/HPTLC/Modified gegen qinlian decoction/Radix et rhizoma glycyrrhizae praeparata cum melle/Radixpuerariae lobatae/Radix scutellariae/Rhizoma coptidis/Rhizoma anemarrhenae/Rhizoma zingiberis/UHPLC-DAD-Q-Exactive-MS引用本文复制引用
Xuehong Nöst,Eva‑Maria Pferschy‑Wenzig,Xiao‑Tong Yu,Min Li,Xiao‑Lin Tong,Rudolf Bauer..Comprehensive Metabolic Profiling of Modified Gegen Qinlian Decoction by Ultra-high-Performance Liquid Chromatography-Diode Array Detection-Q-Exactive-Orbitrap-Electrospray Ionization-Mass Spectrometry/Mass Spectrometry[J].世界中医药杂志（英文版）,2021,7(1):11-32,22.基金项目
The project has been financially supported by the Austrian Federal Ministry of Education,Science and Research(402.000/00012?WF/V/6/2016),Vienna,Austria,by the China Academy of Chinese Medical Sciences as a"Belt and Road"special international cooperation project(GH2017?03?06),Beijing,China,and by the Key Project of the National Natural Science Foundation(No.81430097),Beijing,China.Xiao?Tong Yu acknowledges an Ernst?Mach scholarship by EPU/OeAD,Vienna,Austria. （402.000/00012?WF/V/6/2016）
