首页|期刊导航|世界中医药杂志（英文版）|Differentiation of Belamcandae Rhizoma and Iridis Tectori Rhizoma by Thin-Layer Chromatography and High-Performance Liquid Chromatography
世界中医药杂志（英文版）2021，Vol.7Issue(1)：63-70,8.DOI:10.4103/wjtcm.wjtcm_79_20
Differentiation of Belamcandae Rhizoma and Iridis Tectori Rhizoma by Thin-Layer Chromatography and High-Performance Liquid Chromatography
Differentiation of Belamcandae Rhizoma and Iridis Tectori Rhizoma by Thin?Layer Chromatography and High?Performance Liquid Chromatography
摘要
Abstract
Objective: Belamcandae Rhizoma and Iridis Tectori Rhizoma are easily confused with each other. The main objective of this study is to distinguish them using chemical analysis. Materials and Methods: Thin?layer chromatography (TLC) and high?performance liquid chromatography (HPLC) fingerprint methods were established to compare the chemical profile, while HPLC quantitation was used to determine the contents of three isoflavones in thirty batches of Belamcandae Rhizoma and Iridis Tectori Rhizoma samples. Results: The two herbs could be distinguished by TLC using acetic acid?n hexane?ethyl acetate (1:90:80 v/v/v) as the mobile phase, according to the fluorescent band under 366 nm at Rf 0.2. In total, 12 compounds were identified in the 24?min HPLC fingerprint. The similarity coefficient between the two herbs was 0.54 ± 0.01. Mangiferin (1), tectoridin (2), iridin (3), irigenin (5), irisflorentin (6), and iristectorin A (9) were the main peaks in Belamcandae Rhizoma, while tectoridin (2) and tectorigenin (4) were the major peaks in Iridis Tectori Rhizoma. The contents of 2 in Iridis Tectori Rhizoma (2.50 ± 0.20 ％) were 8.93 times higher than that of Belamcandae Rhizoma (0.28 ± 0.08 ％), while the ones of 5 and 6 were slightly lower in Iridis Tectori Rhizoma. Conclusions: The study established fast and effective methods to distinguish Belamcandae Rhizoma from Iridis Tectori Rhizoma.关键词
Belamcandae Rhizoma/high‑performance liquid chromatography fingerprint/high‑performance liquid chromatographyquantitative analysis/Iridis Tectori Rhizoma/thin‑layer chromatographyKey words
Belamcandae Rhizoma/high‑performance liquid chromatography fingerprint/high‑performance liquid chromatographyquantitative analysis/Iridis Tectori Rhizoma/thin‑layer chromatography引用本文复制引用
Lu‑Lu Xu,Yang Zhang,Yue Chai,Kuan Chen,Hai‑Dong Wang,Chun-Guo Yang,Min Ye,Xue Qiao..Differentiation of Belamcandae Rhizoma and Iridis Tectori Rhizoma by Thin-Layer Chromatography and High-Performance Liquid Chromatography[J].世界中医药杂志（英文版）,2021,7(1):63-70,8.基金项目
This study was supported by the National Key Research and  （）
Development Program of China(No.2018YFC1707904,2018YFC1707900). （No.2018YFC1707904,2018YFC1707900）
