首页|期刊导航|世界中医药杂志（英文版）|Rapid Differentiation of Aconiti Kusnezoffii Radix from Different Geographic Origins Using Ultra-Performance Liquid Chromatography Coupled with Time-of-Flight Mass Spectrometry
世界中医药杂志（英文版）2021，Vol.7Issue(1)：71-77,7.DOI:10.4103/wjtcm.wjtcm_52_20
Rapid Differentiation of Aconiti Kusnezoffii Radix from Different Geographic Origins Using Ultra-Performance Liquid Chromatography Coupled with Time-of-Flight Mass Spectrometry
Rapid Differentiation of Aconiti Kusnezoffii Radix from Different Geographic Origins Using Ultra?Performance Liquid Chromatography Coupled with Time?of?Flight Mass Spectrometry
摘要
Abstract
Objective: There are different geographic origins of Aconiti Kusnezoffii Radixs (AKRs) sold in the market with different quality. This study aims to establish a rapid analysis method to distinguish the different geographic origins of AKRs and to realize the rapid evaluation of their quality. Methods: An ultra?performance liquid chromatography coupled with time?of?flight mass spectrometry (UPLC?Q?TOF MS) method was utilized to acquire the constituents' information of AKRs from different geographic origins. MSE data and Progenesis QI software were employed to identify the chemical constitutes. Principal component analysis (PCA) was applied to comparing MS data to find the chemical markers of AKRs from different geographic origins. Results: Twenty?three components were detected and 17 out of them were identified, including diester?diterpenoid alkaloids, monoester?diterpenoid alkaloids , and amine?diterpenoid alkaloids. Three pairs of isomers were detected and two of them were distinguished by the retention time of standard samples. Thirteen chemical markers were screened out through PCA and orthogonal partial least square discriminant analysis. Through detecting Napelline or isomer of Napelline (m/z 360.2530) and Aconifine (m/z 662.3170), AKRs from inner Mongolia autonomous could be screened. According to the existence of benzoylaconine (m/z 604.3108) and Indaconitine (m/z 630.3159), it could be confirmed that the AKRs are from Xinjiang Uygur autonomous. AKRs that cannot detect compounds above?mentioned could be from Liaoning or Shanxi Province. Conclusions: The chemical profile could be used not only to distinguish the AKRs from different geographic origins but also to identify the true and false of AKRs. This study lays a foundation for the study of efficacy and toxic of AKRs.关键词
Aconiti Kusnezoffii Radix/chemical markers/rapid differentiation/ultra‑performance liquid chromatography coupled withtime‑of‑flight mass spectrometryKey words
Aconiti Kusnezoffii Radix/chemical markers/rapid differentiation/ultra‑performance liquid chromatography coupled withtime‑of‑flight mass spectrometry引用本文复制引用
En‑Yu Lu,Zi‑Feng Pi,Zhong Zheng,Shu Liu,Feng‑Rui Song,Na Li,Zhi‑Qiang Liu..Rapid Differentiation of Aconiti Kusnezoffii Radix from Different Geographic Origins Using Ultra-Performance Liquid Chromatography Coupled with Time-of-Flight Mass Spectrometry[J].世界中医药杂志（英文版）,2021,7(1):71-77,7.基金项目
This research was supported by the National Key Research and Development Project(SQ2018YPC170480,2018YFC1708202),the National Natural Science Foundation of China(81774009)and the Key science and technology  （SQ2018YPC170480,2018YFC1708202）
projects of Inner Mongolia Autonomous Region(2019ZD004),Open Project Program of Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology,China(MDK 2019040). （2019ZD004）
