首页|期刊导航|世界中医药杂志（英文版）|Ultra-High-Performance Liquid Chromatograph with Triple-Quadrupole Mass Spectrometer Quantitation of Twelve Phenolic Components in Different Parts of Sarcandra glabra
Ultra-High-Performance Liquid Chromatograph with Triple-Quadrupole Mass Spectrometer Quantitation of Twelve Phenolic Components in Different Parts of Sarcandra glabra
Jing-Guang Lu Cai-Yun Wang Da-Xin Chen Jing-Rong Wang Kai-Si Che Ming Zhong Wei Zhang Zhi-Hong Jiang
世界中医药杂志（英文版）2021，Vol.7Issue(1)：86-96,11.
世界中医药杂志（英文版）2021，Vol.7Issue(1)：86-96,11.DOI:10.4103/wjtcm.wjtcm_54_20
Ultra-High-Performance Liquid Chromatograph with Triple-Quadrupole Mass Spectrometer Quantitation of Twelve Phenolic Components in Different Parts of Sarcandra glabra
Ultra?High?Performance Liquid Chromatograph with Triple?Quadrupole Mass Spectrometer Quantitation of Twelve Phenolic Components in Different Parts of Sarcandra glabra
摘要
Abstract
Objective: The study objective was to determine phenolic components for the quality control (QC) of cultivated Sarcandra glabra (Thunb.) Makino (S. glabra). Materials and Methods: A sensitive, ultra?high?performance liquid chromatography?tandem mass spectrometric method for the simultaneous determination of 12 phenolic components has been developed. Six caffeoylquinic acids, two caffeoylshikimic acids, and four flavanonol glucosides were selected for the comprehensive analysis of distribution in different parts (root, stem, and leaf). Results: Twelve phenolic components were linear in the concentration range of 0.005–5.0 μg/mL (R2 > 0.995). The relative standard deviation of intra?day and inter?day precision across three validation runs over the entire concentration range was <5％. The recovery determined was within 5％ in terms of relative error. Our results showed that the 12 phenolic compounds were mainly distributed in leaves and stems far more than those in roots. Conclusions: This study provided an ultra-high-performance liquid chromatograph with triple?quadrupole mass spectrometer quantitative method of 12 phenolic components for the QC of cultivated S. glabra. It was found that the phenolic components were significantly accumulated in the aerial parts (stems and leaves) of cultivated S. glabra.关键词
Cultivation/quantitation/Sarcandra glabra/ultra‑high‑performance liquid chromatograph with triple‑quadrupole massspectrometerKey words
Cultivation/quantitation/Sarcandra glabra/ultra‑high‑performance liquid chromatograph with triple‑quadrupole massspectrometer引用本文复制引用
Jing-Guang Lu,Cai-Yun Wang,Da-Xin Chen,Jing-Rong Wang,Kai-Si Che,Ming Zhong,Wei Zhang,Zhi-Hong Jiang..Ultra-High-Performance Liquid Chromatograph with Triple-Quadrupole Mass Spectrometer Quantitation of Twelve Phenolic Components in Different Parts of Sarcandra glabra[J].世界中医药杂志（英文版）,2021,7(1):86-96,11.基金项目
This research was funded by the Science and Technology Development Fund,Macau SAR(File no.0023/2019/AKP)and Guangxi Science and Technology Department Fund(File no.AD17195002). （File no.0023/2019/AKP）
