首页|期刊导航|世界中医药杂志（英文版）|Enzyme-Linked Immunosorbent Assay and Immunochromatographic Strip for Rapid Detection of Atrazine in Three Medicinal Herbal Roots

Enzyme-Linked Immunosorbent Assay and Immunochromatographic Strip for Rapid Detection of Atrazine in Three Medicinal Herbal Roots

Yu‑Dan Wang Jia‑An Qin Liu Wu Bao‑Min Wang Sergei Eremin Shi‑Hai Yang Mei‑Hua Yang

世界中医药杂志（英文版）2021，Vol.7Issue(1)：97-103,7.

世界中医药杂志（英文版）2021，Vol.7Issue(1)：97-103,7.DOI:10.4103/wjtcm.wjtcm_76_20

Enzyme-Linked Immunosorbent Assay and Immunochromatographic Strip for Rapid Detection of Atrazine in Three Medicinal Herbal Roots

Enzyme?Linked Immunosorbent Assay and Immunochromatographic Strip for Rapid Detection of Atrazine in Three Medicinal Herbal Roots

Yu‑Dan Wang ¹Jia‑An Qin ¹Liu Wu ¹Bao‑Min Wang ²Sergei Eremin ³Shi‑Hai Yang ⁴Mei‑Hua Yang²

作者信息

1. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Ministry of Education,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing,China
2. College of Traditional Chinese Medicine,Jilin Agricultural University,Changchun,China
3. College of Agronomy and Biotechnology,China Agricultural University,Beijing,China
4. Department of Chemical Enzymology Faculty of Chemistry,M.V.Lomonosov Moscow State University Leninsky Gory,1,119991 Moscow,Russia
折叠

摘要

Abstract

Objectives: An enzyme?linked immunosorbent assay (ELISA) and colloidal gold?based immunochromatographic (ICG) strip assay will be developed for the rapid and high?throughput detection of atrazine (ATZ) in medicinal herbs. Methods: A monoclonal antibody against ATZ was obtained after the immunization of mice, cell fusion, and hybridoma screening, and the antibody was used to develop direct competitive ELISA (dcELISA) and the ICG strip assay. Results: Both dcELISA and ICG strip methods were established, optimized, and validated for the detection of ATZ in Salviae miltiorrhizae radix et rhizome, Astragali radix, and Isatidis radix. dcELISA had a half?maximum inhibition concentration of 10.56 ng/mL (Salviae miltiorrhizae radix et rhizome), 7.6 ng/mL (Astragali radix), and 8.15 ng/mL (Isatidis radix). The limit of detection (LOD) of the ICG strip assay was 12.5 ng/mL (Salviae miltiorrhizae radix et rhizome), 12.5 ng/mL (Astragali radix), and 6.25 ng/mL (Isatidis radix) in different herb matrices. Due to the recognition characteristics of the monoclonal antibody for the pesticides ATZ, propazine, sebuthylazine, and prometryn, the detection results of real samples by the two immunoassays were confirmed by liquid chromatography–tandem mass spectrometry, which proved the accuracy and reliability of the established methods. Conclusion: The proposed dcELISA and ICG strip methods were suitable for the rapid, convenient, and high?throughput detection of ATZ in these medicinal herbs.

关键词

Astragali radix/atrazine/isatidis radix/monoclonal antibody/salviae miltiorrhizae radix et rhizome

Key words

Astragali radix/atrazine/isatidis radix/monoclonal antibody/salviae miltiorrhizae radix et rhizome

引用本文复制引用

Yu‑Dan Wang,Jia‑An Qin,Liu Wu,Bao‑Min Wang,Sergei Eremin,Shi‑Hai Yang,Mei‑Hua Yang..Enzyme-Linked Immunosorbent Assay and Immunochromatographic Strip for Rapid Detection of Atrazine in Three Medicinal Herbal Roots[J].世界中医药杂志（英文版）,2021,7(1):97-103,7.

基金项目

This work was supported by the National Natural Science Foundation of China(No.81573595) （No.81573595）

CAMS Innovation Fund for Medical Sciences(2017?I2M?1?013) （2017?I2M?1?013）

High-End Foreign Experts Project(No.G20190001644). （No.G20190001644）

世界中医药杂志（英文版）

OACSTPCD

ISSN：2311-8571

访问量0

下载量0

段落导航