首页|期刊导航|世界中医药杂志（英文版）|Simultaneous Determination and Pharmacokinetics of Tetrandrine, Fangchinoline, and Cyclanoline in Rat Plasma by Ultra-High Performance Liquid Chromatography-Mass Spectrometry after Oral Administration of Stephaniae Tetrandrae Radix Extract
Simultaneous Determination and Pharmacokinetics of Tetrandrine, Fangchinoline, and Cyclanoline in Rat Plasma by Ultra-High Performance Liquid Chromatography-Mass Spectrometry after Oral Administration of Stephaniae Tetrandrae Radix Extract
Zhi‑Bin Wang Yue Ma Hua Liu Yu‑Jin Bi Meng Wang Hai‑Xue Kuang
世界中医药杂志（英文版）2021，Vol.7Issue(1)：130-137,8.
世界中医药杂志（英文版）2021，Vol.7Issue(1)：130-137,8.DOI:10.4103/wjtcm.wjtcm_73_20
Simultaneous Determination and Pharmacokinetics of Tetrandrine, Fangchinoline, and Cyclanoline in Rat Plasma by Ultra-High Performance Liquid Chromatography-Mass Spectrometry after Oral Administration of Stephaniae Tetrandrae Radix Extract
Simultaneous Determination and Pharmacokinetics of Tetrandrine, Fangchinoline, and Cyclanoline in Rat Plasma by Ultra?High Performance Liquid Chromatography?Mass Spectrometry after Oral Administration of Stephaniae Tetrandrae Radix Extract
摘要
Abstract
Objective: The objective of the study was to develop a rapid and sensitive ultra?performance liquid chromatography?tandem mass spectrometric method for the determination of tetrandrine, fangchinoline, and cyclanoline in rat plasma and to investigate their pharmacokinetics after oral administration of Stephaniae Tetrandrae Radix extracts. Methods: Sample pretreatment involved methanol pretreatment and liquid–liquid extraction of ethyl acetate from plasma with methanol. Tramadol was used as the internal standard. The analysis was performed using an high strength silica T3 column (100 mm × 2.1 mm, 1.8 μm) and a gradient elution method consisting of mobile phase solution A (0.1％ formic acid in water) and B (acetonitrile) at a flow rate of 0.4 mL/min. The detection was performed using a triple quadrupole tandem mass spectrometer in the multiple reaction monitoring mode and using an electrospray ionization source in the positive ionization mode. Results: High efficiency was achieved with an analysis time of 4 min/sample. The calibration curve linear in the concentration range of 1250 ng/ml (R2 ≥ 0.9900) and the lower limit of quantification is 1 ng/ml. The intraday and interday precision (relative standard deviation) values were lower than 9.4. Accuracy (relative error) was within 10.3％ at all three quality control levels. Conclusions: This method was successfully applied in pharmacokinetics of tetrandrine, fangchinoline, and cyclanoline in rats after oral administration of Stephaniae Tetrandrae Radix extracts. The maximum plasma concentration (Cmax) of tetrandrine, fangchinoline, and cyclanoline was 124.71 ± 16.08, 84.56 ± 3.28, and 57.61 ± 6.26 ng/mL, respectively. The time to reach Cmax was 10.39 ± 3.04 for tetrandrine, 10.17 ± 3.04 for fangchinoline, and 6.40 ± 3.16 for cyclanoline. The pharmacokinetic results might help further guide the clinical application of Stephaniae Tetrandrae Radix.关键词
Pharmacokinetics/rat plasma/stephaniae tetrandrae radix/ultra‑high performance liquid chromatography‑mass spectrometryKey words
Pharmacokinetics/rat plasma/stephaniae tetrandrae radix/ultra‑high performance liquid chromatography‑mass spectrometry引用本文复制引用
Zhi‑Bin Wang,Yue Ma,Hua Liu,Yu‑Jin Bi,Meng Wang,Hai‑Xue Kuang..Simultaneous Determination and Pharmacokinetics of Tetrandrine, Fangchinoline, and Cyclanoline in Rat Plasma by Ultra-High Performance Liquid Chromatography-Mass Spectrometry after Oral Administration of Stephaniae Tetrandrae Radix Extract[J].世界中医药杂志（英文版）,2021,7(1):130-137,8.基金项目
This study was financially supported by National Natural Science Foundation of China/81973439 （）
Heilongjiang University of Chinese Medicine Research Fund/201504,National Natural Science Foundation of China/81803686 （）
Research Fund of Heilongjiang University of Traditional  （）
Chinese Medicine/201504,Heilongjiang Postdoctoral Research Start?up Funding Project/LBH?Q16214 （）
Heilongjiang Science Foundation Project/H2018056 （）
Heilongjiang University of Traditional Medicine Talents Support Plan/2018RCD03. （）
