中国生态农业学报（中英文）2021，Vol.29Issue(5)：870-879,10.DOI:10.13930/j.cnki.cjea.200888
基于快速叶绿素荧光参数的不同基因型棉花叶片衰老研究
The rapid chlorophyll a fluorescence characteristics of different cotton genotypes reflect differences in leaf senescence
摘要
Abstract
The light reactions of photosystem Ⅱ (PSⅡ) is greatly associated with the photosynthetic capacity. In order to capture more detailed information describing the status of PSⅡ during leaf senescence and rapidly screen cotton (Gossypium L.) genotypes with different duration of photosynthetic capacity, the PSⅡ photochemistry of the first leaves counted from the stem top of three cotton genotypes ('Baimian1' 'Baimian5' and 'DP99B') presented different leaf senescence progresses in production were examined by chlorophyll a fluorescence (Chl F) analysis during leaf senescence. The results showed that 'Baimian1' 'Baimian5' and 'DP99B' were late, intermediate and early aging types, respectively, based on the performance index of light absorption (PIABS). The three genotypes complied with the similar patterns in electrical transferring inhibition accompanying leaf senescence. The depletion of oxygen-evolving complex (OEC) was obvious at the late growth stage. The inhibition of the acceptor side of PSⅡ was greater than that of the donor side. The electron flow that through the light reactions of photosystem Ⅱ and photosystem Ⅰwas significantly limited accompanying leaf senescence. With the duration of leaf senescence, the energy distributed to thermal dissipation and the primary quinone electron acceptors of PSⅡ (QA) restoration increased, and correspondingly the energy used to transport an electron into the electron transport chain beyond AQ- (the reduction state of QA) declined. However, three cotton genotypes showed greater and greater electron transferring inhibition, except the number of QA reduction events between time=0 and time to reach maximal fluorescence, in the order of 'DP99B'>'Baimian5'>'Baimian1' with the duration of leaf senescence. It can be seen that the chlorophyll fluorescence characteristics can quickly and noninvasively reflect the senescence and the internal physiological mechanism of leaf senescence among different cotton genotypes.关键词
棉花/叶片衰老/叶绿素荧光/光系统Ⅱ(PSⅡ)/快速叶绿素荧光参数Key words
Cotton/Leaf senescence/Chlorophyll a fluorescence/Photosystem Ⅱ/Rapid chlorophyll a fluorescence parameter分类
农业科技引用本文复制引用
薛惠云,王素芳,张新,张志勇..基于快速叶绿素荧光参数的不同基因型棉花叶片衰老研究[J].中国生态农业学报（中英文）,2021,29(5):870-879,10.基金项目
This study was supported by the Program for Innovative Research Team(in Science and Technology)in University of Henan Province(21IRTSTHN023)and the National Natural Science Foundation of China(31571600,31271648).河南省高校科技创新团队支持计划项目(21IRTSTHN023)和国家自然科学基金项目(31571600,31271648)资助 （in Science and Technology）
