东南大学学报（英文版）2021，Vol.37Issue(1)：1-7,7.DOI:10.3969/j.issn.1003-7985.2021.01.001
基于双热电偶差值法的细胞温度测量
Intracellular temperature measurement by a dual-thermocouple difference method
摘要
Abstract
A dual thermocouple difference technique is developed to determine the accuracy and anti-interference ability in the process of intracellular temperature measurement.First,two micro-nano thermocouples(TC)and a high-precision signal acquisition module are used to measure the temperature difference between the cell and the culture medium(separated about 10 μm from the cell).The cold junctions of two TCs are connected to eliminate the setting of the reference temperature and enhance the anti-interference ability.Then,a low-noise voltage amplifier and digital acquisition card are used to sample signals.In order to verify the feasibility of the dual thermocouple difference method,the temperature changes of U251 cells are detected.The calibration results of two TCs show that the Seebeck coefficient is about 5 μV/℃,and the signal acquisition accuracy is 0.5 μV in a low voltage range(0-15 μV).With the dual thermocouple difference method,errors due to the cold junctions can be removed and the interference caused by environmental temperature fluctuation can be reduced.The phenomenon of cellular temperature increase proves that the dual thermocouple difference method can detect the tiny temperature change of a single cell.The method potentially is a highly powerful technique for studying local thermogenesis of cells and helps to explore the relationship between cellular thermogenesis and cellular processes.关键词
双热电偶/差温法/细胞内温度/纳米技术Key words
dual-thermocouple/difference thermometry/intracellular temperature/nanotechnology分类
生物科学引用本文复制引用
韩樾夏,何伟男,杨升,毛伟,顾宁..基于双热电偶差值法的细胞温度测量[J].东南大学学报（英文版）,2021,37(1):1-7,7.基金项目
The National Key Research and Development Pro-gram of China(No.2017YFA0104302),the National Natural Science Foundation of China(No.61420106012,61821002). （No.2017YFA0104302）
