广西医科大学学报2021，Vol.38Issue(4)：627-635,9.DOI:10.16190/j.cnki.45-1211/r.2021.04.001
长链非编码RNA NKILA调节miR-889-3p介导肝细胞癌进展的研究
Long non-coding RNA NKILA mediates the progression of hepatocellular carcinoma through regulating miR-889-3p
摘要
Abstract
Objective:To explore the potential role of NKILA in the process of hepatocellular carcinoma(HCC)develop-ment.Methods:The relative expression of NKILA and miR-889-3p in HCC tissues and cells was quantified by quantitative real-time reverse transcription PCR(qRT-PCR).Cell growth and tumor angiogenesis were assessed by colony formation and angiogenesis assay,respectively.Western blotting was used to detect epithelial-mesenchymal transition(EMT)-relat-ed proteins.The association between NKILA and miR-889-3p was conducted by luciferase reporter analysis.A model of mouse xenograft tumor was established to detect the expres-sion of related proteins by immunohistochemistry(IHC)analy-sis.Results:miR-889-3p was a direct target of NKILA,and NKILA negatively regulated miR-889-3p.qRT-PCR assay showed the NKILA expression was conspicuously down-regu-lated and miR-889-3p expression was up-regulated in HCC tis-sues and cells.NKILA overexpression suppressed HCC cell proliferation,EMT and tumor angiogenesis through directly sponging miR-889-3p.More importantly,in vivo model dis-played NKILA significantly reduced the volume and weight of xenograft tumor,accompanied by E-cadherin increase and VEGF decrease.Furthermore,IHC revealed NKILA alone re-duced the number of vascular endothelial cell markers(CD34 and CD 105).Conclusion:NKILA overexpression curbed the development of HCC via regulating miR-889-3p,which may be a novel therapeutic target in patients with HCC.关键词
长的非编码RNA/恶性肿瘤/上皮-间质转化/肿瘤血管生成Key words
long non-coding RNA/malignant neoplasm/epi-thelial-mesenchymal transformation/tumor angiogenesis分类
医药卫生引用本文复制引用
王红梅,陈思瑞,杜红,曹佳..长链非编码RNA NKILA调节miR-889-3p介导肝细胞癌进展的研究[J].广西医科大学学报,2021,38(4):627-635,9.基金项目
Acknowledgments This work was supported by a grant from the Key projects of science and Technolo-gy Department of Sichuan Province(No.2019SZ0069) （No.2019SZ0069）
