水生生物学报2021，Vol.45Issue(3)：495-506,12.DOI:10.7541/2021.2020.036
敲除trim47基因斑马鱼脑和脾的比较转录组学分析
COMPARATIVE TRANSCRIPTOMIC ANALYSIS OF BRAIN AND SPLEEN IN TRIM47 KNOCKOUT ZEBRAFISH (DANIO RERIO)
摘要
Abstract
Tripartite motif (TRIM) proteins is a structurally conserved protein group that plays an important role in many biological processes, such as apoptosis, cycle regulation, cellular responses to viruses and in-flammatory response. Trim47 is a member of the TRIM family. In this study, the CRISPR/Cas9 technique was employed to knockout trim47 of zebrafish. Brain and spleen of TRIM47–/–(trim47 knockout) and WT (wildtype) zebrafish were collected for RNA-seq analysis to identify differentially expressed genes (DEGs). A total of 271 DEGs were identified. After a brief analysis, these DEGs were annotated into Kyoto Encyclo-pedia of Genes and Genomes (KEGG) pathway and three ontologies following gene ontology (GO) enrich-ment analysis. DEGs were more concentrated in cell adhesion and glutamatergic synapse signaling pathway in brain of TRIM47–/– group as compared with WT group. In spleen, DEGs of TRIM47–/– group changed in complement and coagulation cascades signaling pathway as compared with WT group. The complement path-way-associated genes in brain and spleen were verified using qRT-PCR, which were consistent with the tran-scriptome data. These results indicated that Trim47 played an important biological role in spleen and brain, particularly involved in innate immune function through complement pathway. In vivo infection experiments showed that trim47 knockout increased the infection rate of Spring Viremia of Carp Virus (SVCV) in ze-brafish. In conclusion, these findings provided new insight into the function of the TRIM members in innate immunity.关键词
TRIM47/敲除/差异基因/斑马鱼Key words
TRIM47/Knockout/DEGs/Zebrafish分类
生物科学引用本文复制引用
姚健,王业大,王方,柳力月,鲁元安,刘学芹..敲除trim47基因斑马鱼脑和脾的比较转录组学分析[J].水生生物学报,2021,45(3):495-506,12.基金项目
Supported by the National Natural Science Foundation of China(31972834) （31972834）
the National Key Research and Development Program of China(2018YFD0900505) （2018YFD0900505）
Fundamental Research Funds for the Central Universities(2662018YJ022) （2662018YJ022）
