中国药理学与毒理学杂志2021，Vol.35Issue(3)：169-175,7.DOI:10.3867/j.issn.1000-3002.2021.03.002
柴胡皂苷d对H2O2诱导的肝细胞损伤的保护作用及其机制
Protective effect of saikosaponin-d on H2O2-induced hepatocellular injury and its mechanism
摘要
Abstract
OBJECTIVE To investigate the effects of saikosaponin-d (SSd) on the proliferation of L02 cells induced by H2O2 and the mechanism. METHODS ① L02 cells were pretreated with SSd 0.5, 1 and 2μmol·L-1 for 6 h, and administered with H2O2800μmol·L-1 for 24 h. Cell viability was analyzed by MTT assay, while the activities of glutamic-oxalacetic transaminase (GOT), glutamic-pyruvic transam?inase (GPT), as well as the contents of malondialdehyde (MDA), and lactate dehydrogenase (LDH) in cell culture were detected by biochemical assay.②L02 cells were pretreated with SSd 2μmol·L-1 and tert-butylhydroquinone (tBHQ) 50 μmol · L-1 for 6 h, and administered with H2O2800 μmol · L-1 for 24 h. The intracellular accumulation of reactive oxygen species (ROS) was analyzed by fluorescent probes, nuclear translocation of nuclear factor-erythroid 2-related factor 2 (Nrf2) was determined by immunoflu?orescence assay, the protein expressions of Nrf2 and heme oxygenase-1 (HO-1) were detected by Western blotting. RESULTS ①Cell survival rate was decreased in model group significantly (vs control, P<0.05), but increased in H2O2+SSd 1 and 2μmol·L-1 group significantly (vs H2O2, P<0.05). The activi?ties of GPT, GOT and the contents of MDA and LDH were significantly increased in model group (P<0.05). Compared with model group, the activities of GPT, GOT and the contents of MDA and LDH were significantly decreased in H2O2+SSd 2 μmol · L-1 (vs H2O2, P<0.05). ② H2O2 significantly increased ROS production (vs cell control, P<0.05), in H2O2+SSd 2 μmol · L-1 group, but the ROS levels were significantly reduced (vs H2O2, P<0.05). Compared with the cell control group, the localization of Nrf2 in the nucleus was increased intuitively in SSd 2 μmol · L-1 and tBHQ 50 μmol · L-1 group. Compared with H2O2 group, the protein expressions of Nrf2 and HO-1 in H2O2+SSd 2μmol·L-1 or H2O2+tBHQ 50μmol·L-1 group were enhanced (all P<0.05). SSd 2 μmol · L-1 alone could significantly increase the expressions of Nrf2 and HO-1 in L02 cells (vs cell control and H2O2, P<0.05). CONCLUSION SSd can effectively protect against L02 cell injury induced by H2O2 treatment, and the mechanism of which is related to the decrease of intracellular ROS, the up-regulation of Nrf2 and HO-1 protein expression.关键词
柴胡皂苷-d/非酒精性脂肪性肝病/核因子E2相关因子2/血红素氧合酶1/氧化应激Key words
saikosaponin-d/non-alcoholic fatty liver disease/nuclear factor-erythroid 2-related factor 2/heme oxygenase-1/oxidative stress分类
医药卫生引用本文复制引用
黄小凤,刘泽干,雷攀,孟忠吉,王莉博,杜士明..柴胡皂苷d对H2O2诱导的肝细胞损伤的保护作用及其机制[J].中国药理学与毒理学杂志,2021,35(3):169-175,7.基金项目
Small and Medium-Sized Enterprises Technology Innovation Fund(10c26214204534) （10c26214204534）
Open Project of Hubei Key Laboratory of Wudang Local Chinese Medicine Research(Hubei University of Medicine)(WDCM2019015) （Hubei University of Medicine）
and Guided Research Projects of Shiyan Science and Technology Bureau(19Y24)中小企业科技创新基金(10c26214204534) （19Y24）
武当特色中药研究湖北省重点实验室(湖北医药学院)开放课题(WDCM2019015) （湖北医药学院）
十堰引导性科研项目(19Y24) （19Y24）
