数字中医药（英文）2021，Vol.4Issue(2)：120-129,10.DOI:10.1016/j.dcmed.2021.06.005
不同产地白芍与赤芍的氨基酸特征谱表征研究
Evaluation of Baishao (Paeoniae Radix Alba) and Chishao (Paeoniae Radix Rubra) from different origins based on characteristic spectra of amino acids
摘要
Abstract
Objective To evaluate the difference between Baishao (Paeoniae Radix Alba, PRA) and Chishao (Paeoniae Radix Rubra, PRR) from different regions based on the characteristic spectra of amino acids (AAs). Methods Fingerprints of the 21 standard AAs were established using O-phthalaldehyde-9-fluorenylmethyl chloroformate (OPA-FMOC) pre-column derivation method. The AA components in PRA and PRR were characterized qualitatively and quantitatively, and different AAs were screened using pattern recognition technology. Results Twelve AAs were identified in both PRA and PRR. Meanwhile, aspartic acid, glutamic acid, arginine, alanine, and gamma-aminobutyric acid were screened as characteristic components, and their concentrations could effectively distinguish PRA from PRR. Conclusion The established characterization method, which is based on the characteristic spectra of AAs, is accurate, efficient, and sensitive and can effectively distinguish between PRA and PRR from different producing areas, thus providing a reference for the overall characterization and evaluation of Chinese medicinal materials.关键词
OPA-FMOC柱前衍生法/氨基酸特征谱/白芍/赤芍/模式识别Key words
O-phthalaldehyde-9-fluorenylmethyl chloroformate (OPA-FMOC) pre-column derivatization method/Amino acids characteristic spectra/Baishao (Paeoniae Radix Alba, PRA)/Chishao (Paeoniae Radix Rubra, PRR)/Pattern recognition引用本文复制引用
李莎莎,张怡萍,邓远辉,王倩,谢志茹,Ibarra-Estrada Emmanuel,肖雪..不同产地白芍与赤芍的氨基酸特征谱表征研究[J].数字中医药（英文）,2021,4(2):120-129,10.基金项目
We thank for the funding support from the Guang-dong Provincial Key Laboratory of Chinese Medicine for Prevention and Treatment of Refractory Chronic Diseases(No.2018B030322012). （No.2018B030322012）
