首页|期刊导航|畜牧与生物技术杂志(英文版)|Effects of maternal dietary omega-3 polyunsaturated fatty acids and methionine during late gestation on fetal growth,DNA methylation,and mRNA relative expression of genes associated with the inflammatory response, lipid metabolism and DNA methylation
Effects of maternal dietary omega-3 polyunsaturated fatty acids and methionine during late gestation on fetal growth,DNA methylation,and mRNA relative expression of genes associated with the inflammatory response, lipid metabolism and DNA methylation
Milca Rosa Velazquez Fernanda Batistel Juan Manuel Pinos Rodriguez Alejandro Enrique Relling
畜牧与生物技术杂志(英文版)2021，Vol.12Issue(2)：611-625,15.
畜牧与生物技术杂志(英文版)2021，Vol.12Issue(2)：611-625,15.
Effects of maternal dietary omega-3 polyunsaturated fatty acids and methionine during late gestation on fetal growth,DNA methylation,and mRNA relative expression of genes associated with the inflammatory response, lipid metabolism and DNA methylation
摘要
Abstract
Background:Omega-3 PUFA or methionine (Met) supply during gestation alters offspring physiology. However, the effect of both nutrients on fetal development has not been explored. Our objective was to determine the effects of supplementation of these two nutrients during late gestation on fetal growth, DNA methylation, and mRNA expression of genes associated with the inflammatory response, and DNA methylation. Ewes (n=5/treatment) were fed from day 100 to 145 of gestation one of the following treatments:1) basal diet (NS) without fatty acids (FS) or methionine (MS) supplementation;2) FS (10 g/kg Ca salts, source omega-3 PUFA);3) MS (1 g/kg rumen protected methionine);and 4) FS and MS (FS-MS). On day 145, ewes were euthanized, and data from dams and fetus was recorded. Placenta (cotyledon), fetal liver, and blood samples were collected. (Continued on next page) (Continued from previous page) Results:A treatments interaction on fetal liver weight, ewe body weight and body condition score (BCS) was observed;FS-MS were heavier (P< 0.01) than FS and MS, and FS-MS ewes had a better (P=0.02) BCS than NS. Methionine increased (P=0.03) ewe plasma glucose concentration. Fetal liver global DNA methylation increased (P< 0.01) in FS and MS. Dietary treatments modify the mRNA relative expression on some of the genes evaluated. In the fetal liver, FS increased (P=0.04) the mRNA relative expression of arachidonate-5-lipoxygenase-activating-protein and tended to decrease (P=0.06) methionine-adenosyltransferase-1A. Moreover, MS decreased (P=0.04) DNA-methyltransferase-1 and tended to decrease (P=0.08) free-fatty-acid-receptor-1 mRNA relative expression. Furthermore, FS-MS decreased mRNA relative expression of tumor-necrosis-factor-alpha (P=0.05), peroxisome-proliferator-activated-receptor-delta (P=0.03) and gamma (P=0.04), tended to decrease (P≤0.09) interleukin-6, fatty-acid-transport-protein-1, and delta-5-desaturase, and increased adenosylhomocysteinase (P=0.04) mRNA relative expression. In cotyledon, FS tended to decrease fatty acid binding protein 4 (P=0.09) mRNA relative expression. Conclusion:Omega-3 PUFA and Met supplementation improves dam's performance in late gestation, which was positively correlated with an increase in offspring's liver development. Moreover, FS-MS decreased mRNA relative expression of proinflammatory cytokines, and lipogenic genes, and increased the expression on an enzyme that has an important role in methylation.关键词
Fat supplementation/Fetal programming/Maternal nutrition/Nutrients/Rumen protected methionineKey words
Fat supplementation/Fetal programming/Maternal nutrition/Nutrients/Rumen protected methionine引用本文复制引用
Milca Rosa Velazquez,Fernanda Batistel,Juan Manuel Pinos Rodriguez,Alejandro Enrique Relling..Effects of maternal dietary omega-3 polyunsaturated fatty acids and methionine during late gestation on fetal growth,DNA methylation,and mRNA relative expression of genes associated with the inflammatory response, lipid metabolism and DNA methylation[J].畜牧与生物技术杂志(英文版),2021,12(2):611-625,15.基金项目
This research did not receive any monetary funds from funding agencies in the public,commercial,or not-for-profit sectors.The fatty acid and methio-nine supplement were donated by Virtus Nutrition and Adisseo,respectively. （）
