首页|期刊导航|世界中医药杂志（英文版）|Pharmacokinetic and Relative Bioavailability of Three Secoiridoid Glycosides in Beagle Dog Plasma After Oral Administration of Conventional and Enteric-Coated Capsules of Gentianella acuta Extract
Pharmacokinetic and Relative Bioavailability of Three Secoiridoid Glycosides in Beagle Dog Plasma After Oral Administration of Conventional and Enteric-Coated Capsules of Gentianella acuta Extract
Zhi-Bin Wang Kai Li Meng Wang Gao-Song Wu Yu-Jin Bi Hai-Xue Kuang
世界中医药杂志（英文版）2021，Vol.7Issue(2)：246-253,8.
世界中医药杂志（英文版）2021，Vol.7Issue(2)：246-253,8.DOI:10.4103/wjtcm.wjtcm_10_21
Pharmacokinetic and Relative Bioavailability of Three Secoiridoid Glycosides in Beagle Dog Plasma After Oral Administration of Conventional and Enteric-Coated Capsules of Gentianella acuta Extract
Pharmacokinetic and Relative Bioavailability of Three Secoiridoid Glycosides in Beagle Dog Plasma After Oral Administration of Conventional and Enteric?Coated Capsules of Gentianella acuta Extract
摘要
Abstract
Objective: To simultaneously investigate the pharmacokinetics of gentiopicroside, sweroside, and swertiamarin, which are constituents of Gentianella acuta, by developing and validating a simple, sensitive, and fast ultra?high?performance liquid chromatography–tandem mass spectrometry method. Materials and Methods: Blood samples were collected from the forward limb veins of six beagle dogs following oral gavage with G. acuta, the whole plant extract (39.90 mg/kg). Plasma samples were processed using liquid–liquid extraction. The analytes and paeoniflorin (internal standard [IS]) were separated using an Acquity? UPLC ethylene bridged hybrid amide column (2.1 mm × 100 mm, 1.7 μm) with isocratic elution using a mobile phase consisting of acetonitrile and 0.1％ formic acid in water (80:20, v/v) at a flow rate of 0.4 mL/min. Quantification was performed using multiple reaction monitoring of the fragmentation transitions at m/z 401.1→179.0, 403.1→195.0, 419.1→179.0, and 525.2→449.1 for gentiopicroside, sweroside, swertiamarin, and the IS, respectively. Results: The linearity of the analytical response was good and the calibration curves were linear over concentration ranges of 1.20–192.0, 0.40–159.0, and 0.20–209.3 ng/mL for gentiopicroside, sweroside, and swertiamarin, respectively. The extraction recovery was in the range of 84.72％–91.34％, 84.58％–93.43％, and 82.75％–91.37％ for gentiopicroside, sweroside, and swertiamarin, respectively. Conclusions: The method was successfully used to evaluate the pharmacokinetic parameters of gentiopicroside, sweroside, and swertiamarin in beagle dogs.关键词
Beagle dog/Gentianella acuta/pharmacokinetics/secoiri doid glycosides/ultra‑high‑performance liquid chromatography–tandem mass spectrometryKey words
Beagle dog/Gentianella acuta/pharmacokinetics/secoiri doid glycosides/ultra‑high‑performance liquid chromatography–tandem mass spectrometry引用本文复制引用
Zhi-Bin Wang,Kai Li,Meng Wang,Gao-Song Wu,Yu-Jin Bi,Hai-Xue Kuang..Pharmacokinetic and Relative Bioavailability of Three Secoiridoid Glycosides in Beagle Dog Plasma After Oral Administration of Conventional and Enteric-Coated Capsules of Gentianella acuta Extract[J].世界中医药杂志（英文版）,2021,7(2):246-253,8.基金项目
This work was supported by the Research Project of Heilongjiang University of Chinese Medicine"Supporting Plan for Excellent Innovative Talents"(2018RCD03),Heilongjiang Provincial Science Fund Project(H2018056),Heilongjiang Post?doctoral Research Start Fund Project(LBH?Q16214),General Projects of NSFC(81973439,81872979,and 81803686),and Research Fund of Heilongjiang University of Chinese Medicine(201504). （2018RCD03）
