广西医科大学学报2021，Vol.38Issue(6)：1067-1076,10.DOI:10.16190/j.cnki.45-1211/r.2021.06.001
Musashi2基因在肺鳞癌中的表达及其对Notch1信号通路介导的CD44v6(+)肺癌干细胞维持的研究
Musashi2 gene expression in lung squamous cell carcinoma and its effect on the maintenance of CD44v6(+) lung cancer stem cells mediated by N0tch1 signaling pathway
摘要
Abstract
Objective:To study the effect of Musashi2 gene on the sternness of CD44v6(+) lung cancer stem cells and ex-plore the mechanism.Methods:Fifty clinical lung squamous cell carcinoma tissues and adjacent tissues were collected.The expression of Musashi2 and CD44v6 was detected by qRT-PCR.CD44v6(+) and CD44v6(-) SK-MES-1 cells were sorted using the microbead sorting method.CD44v6(-) cells were di-vided into pcDNA-Musashi2 group and pcDNA-NC group;CD44v6(+) cells were divided into control group and Notchl inhibitor RO4929097 group.CD44v6(-) cells transfected with pcDNA-Musashi2 were divided into pcDNA-Musashi2 group and pcDNA-Musashi2+RO4929097 group.The sphere forma-tion experiment was used to detect the self-renewal ability of the cells in each group.Western blotting was performed to de-tect the protein expressions of stem cell markers Nanog,Oct4,Sox2,Notchl,Hey1,and Musashi2.Results:The expressions of Musashi2 and CD44v6 in lung squamous cell carcinoma tis-sues were higher than those in adjacent tissues (P＜0.05).Compared with CD44v6(-) cells,the spheroidization ability of CD44v6(+) cells was enhanced (P＜0.05),and the expressions of Musashi2,Nanog,Oct4,Sox2,Notch1 and Hey1 were up-regulated (all P＜0.05).Compared with pcDNA-NC,the pcD-NA-Musashi2 group had enhanced spheroidizing ability (P＜0.05),and the expressions of Musashi2,Nanog,Oct4,Sox2,Notch1 and Hey1 were up-regulated (P＜0.05).Compared with the control group,the spheroidization ability of RO4929097 group was reduced (P＜0.05),and the expressions of Musashi2,Nanog,Oct4,Sox2,Notch1 and Hey1 were down-regulated (P＜0.05),Compared with the pcDNA-Musashi2 group,the pcDNA-Musashi2 + RO4929097 group had lower spheroidizing ability (P＜0.05),and the expressions of Musashi2,Nanog,Oct4,Sox2,Notch1 and Hey1 were down-regulated (P＜0.05).Conclusion:Musashi2 maintains the stemness of CD44v6(+) lung cancer stem cells through Notch1 signaling.关键词
Musashi2/CD44v6/Notch1信号通路/肿瘤干细胞/肺鳞癌Key words
Musashi2/CD44v6/Notch1 signaling pathway/cancer stem cells/lung squamous cell carcinoma分类
医药卫生引用本文复制引用
张曦,雷光焰,张新伟..Musashi2基因在肺鳞癌中的表达及其对Notch1信号通路介导的CD44v6(+)肺癌干细胞维持的研究[J].广西医科大学学报,2021,38(6):1067-1076,10.基金项目
This study was developed by Key Research and Development Program of Shaanxi(No.2019SF-319). （No.2019SF-319）
