热带亚热带植物学报2021，Vol.29Issue(4)：339-348,10.DOI:10.11926/jtsb.4315
马尾松PmPGK1和PmGPIC基因的克隆和表达分析
Cloning and Expression Analysis on PmPGK1 and PmGPIC Genes in Pinus massoniana
摘要
Abstract
To understand the functions of phosphoglycerate kinase 1 (PGK1) and cytosolic glucose phosphate isomerase (CPIC) of Pinus massoniana, the cDNA of PmPGK1 and PmGPIC were cloned by RACE, and the bioinformatic and subcellular localization of PmPGK1 and PmGPIC were analyzed, and then their expression patterns were performed by qRT-PCR. The results showed that the full-length cDNA of PmPGK1 and PmGPIC were 2106 and 1848 bp, encoding 507 and 566 amino acids, respectively. PmPGK1 and PmGPIC proteins were located in chloroplast and cytosol, respectively. The expression of PmPGK1 was in order of new leaf>old leaf> new stem>root>flower, while that of PmGPIC was old leaf>flower>new leaf>new stem>root. Under low temperature stress for 24 hours, the expression of PmPGK1 and PmGPIC decreased at first and then increased, and the expression of PmGPIC decreased to a low level after 2 hours. Under high CO2 stress for 24 hours, the expression of PmPGK1 was significantly down-regulated, showing a trend of decrease-increase-decrease, while the down-regulation of PmGPIC was not obvious. Therefore, it was suggested that PmPGK1 mainly participated in the Calvin cycle and chloroplast/plast glycolysis, and PmGPIC was mainly involved in cytosolic glycolysis. The activities of PmPGK1 and PmGPIC were inhibited under low temperature stress, and PmPGK1 activity was significantly inhibited under high CO2 stress, while PmGPIC activity was less affected.关键词
马尾松/PmPGK1/PmGPIC/基因克隆/亚细胞定位Key words
Pinus massoniana/PmPGK1/PmGPIC/Gene clone/Subcellular localization引用本文复制引用
夏林超,吴帆,季孔庶..马尾松PmPGK1和PmGPIC基因的克隆和表达分析[J].热带亚热带植物学报,2021,29(4):339-348,10.基金项目
This work was supported by the"Thirteenth-five"National Key Research and Development Program of China(Grant No.2017YFD0600304),and the Program for Priority Academy Development of Jiangsu Higher Education Institutions(PAPD). （Grant No.2017YFD0600304）
