广西医科大学学报2021，Vol.38Issue(7)：1243-1253,11.DOI:10.16190/j.cnki.45-1211/r.2021.07.001
CTRP3通过激活SIRT1/FOXO3a途径减轻OGD/R诱导的心肌细胞损伤
CTRP3 alleviates OGD/R-induced myocardial cell injury by activating the SIRT1/Fox03a pathway
摘要
Abstract
Objective:To explore the role of CTRP3 in cardio-myocyte injury induced by oxygen glucose deprivation/reperfu-sion(OGD/R)and its possible mechanism.Methods:Rat car-diomyocytes H9c2 were cultured in vitro,and OGD/R induced H9c2 cell injury.qRT-PCR was used to detect the expression of CTRP3,SIRT1 and FOXO3a mRNA in cells.Western blot was used to detect the expression of CTRP3,SIRT1,FOXO3a,Bax,Bcl-2 and cleaved caspase-3 protein in cells.CCK-8 was used to detect cell survival.Flow cytometry was used to detect cell apoptosis.The kit was used to detect the CK-MB,cTnl,SOD and MDA activity,and the GSH-Px and ROS production of cells.Results:Compared with the control group,the expres-sions of CTRP3,SIRT1 and FOXO3a mRNA and protein,the cell survival rate,Bcl-2 protein expression,and SOD and GSH-Px activities were significantly reduced(P＜0.05),while the apoptosis rate,the expression of Bax and cleaved caspase-3 protein,the contents of CK-MB,cTnI and MDA,and ROS flu-orescence intensity were significantly increased in OGD/Rgroup(P＜0.05).There were no significant differences in cell indicators in the oe-NC group and OGD/R group(P＞0.05).Compared with the oe-NC group,the CTRP3,SIRT1.and FOXO3a mRNA and protein expressions,cell survival rate,Bcl-2 protein expression,and SOD and GSH-Px activities were significantly increased,whereas the apoptosis rate,the ex-pression of Bax and cleaved caspase-3 protein,the contents of CK-MB,cTnI and MDA,and ROS fluorescence intensity were significantly reduced in the oe-CTRP3 group(P＜0.05).Com-pared with the oe-CTRP3 group,the SIRT1,FOXO3a and Bcl-2 protein expression,cell survival rate,SOD and GSH-Px ac-tivities in the oe-CTRP3+EX527 group were significantly re-duced,while the apoptosis rate,the expression of Bax and cleaved caspase-3 protein,the contents of CK-MB,cTnI and MDA,and ROS fluorescence intensity were increased signifi-cantly(P＜0.05).Compared with the oe-CTRP3+EX527 group,the FOXO3a and Bcl-2 protein expressions,the cell sur-vival rate and SOD and GSH-Px activities were significantly increased,while the apoptosis rate,the expressions of Bax and cleaved caspase-3 protein,the contents of CK-MB,cTnl and MDA,and ROS fluorescence intensity were significantly re-duced in the oe-CTRP3+EX527+oe-FOXO3a group(P＜0.05).Conclusion:CTRP3 promotes the survival of cardiomyocytes induced by OGD/R and inhibits apoptosis and oxidative stress by activating the SIRTl/FOXO3a pathway.关键词
心肌细胞/糖氧剥夺/再灌注/CTRP3/SIRT1/FOXO3aKey words
cardiomyocytes/glucose and oxygen deprivation/reperfusion/CTRP3/SIRT1/FOXO3a分类
医药卫生引用本文复制引用
贺丹娜,赵瑞平,宋秀荣,李帷,胡君,卢耀军..CTRP3通过激活SIRT1/FOXO3a途径减轻OGD/R诱导的心肌细胞损伤[J].广西医科大学学报,2021,38(7):1243-1253,11.基金项目
This study was funded by Nation-al Natural Science Foundation of China(No.81760077) （No.81760077）
