农业生物技术学报2021，Vol.29Issue(9)：1649-1664,16.DOI:10.3969/j.issn.1674-7968.2021.09.001
铁皮石斛PIN基因家族的全基因组鉴定和表达分析
Genome-wide Identification and Expression Analysis of PIN Gene Family in Dendrobium catenatum
摘要
Abstract
Auxin export carrier PIN-FORMED (PIN) family members mainly mediate auxin polar transport and asymmetric distribution. To better understand Dendrobium catenatum PIN (DcPIN) gene family, the present study performed genome-wide identification of DcPIN members, and further analyzed their phylogenetic relationships, gene structure, protein conserved motifs and domain composition, promoter cis-acting element, and expression profiles of both tissues and stress responses by means of bioinformatics and transcriptome data. The results showed that D. catenatum contained 21 DcPIN members that were divided into 7 clades. Among them, PIN9 clade was specific to monocot and contained high number of DcPIN9 genes in D. catenatum, which might be strongly relevant to its unique lifestyle and living environment. The upstream DcPIN promoters were rich in cis-regulatory elements related to hormones, light, development, and stress response. Transcriptome data showed the expression of DcPIN genes in different tissues could be totally divided into specific expression and constitutive expression. Transcriptome and qRT-PCR analysis under different treatments showed DcPIN1a/9a/9d/9g were in response to cold, DcPIN9a/9f/9h in response to drought, DcPIN3a/9a/9d/9h in response to JA, and DcPIN1c/3a/9a in response to the infection of Southern Blight pathogen Sclerotium delphinii. This result provides a theoretical basis for further investigating the function of D. catenatum PIN genes in the growth and development and environmental adaptation.关键词
铁皮石斛/PIN基因家族/生长素/极性运输/基因表达Key words
Dendrobium catenatum/PIN gene family/Auxin/Polar transport/Gene expression分类
农业科技引用本文复制引用
王灏,姜伟伟,郭英纯,斯金平,陈东红..铁皮石斛PIN基因家族的全基因组鉴定和表达分析[J].农业生物技术学报,2021,29(9):1649-1664,16.基金项目
国家重点研发计划(2017YFC1702201) （2017YFC1702201）
国家自然科学基金(31870310) （31870310）
浙江农林大学科研发展基金人才启动项目(2017FR035) （2017FR035）
省部共建亚热带森林培育国家重点实验室自主研究课题(ZY20180206) （ZY20180206）
