中国病理生理杂志2021，Vol.37Issue(8)：1438-1446,9.DOI:10.3969/j.issn.1000-4718.2021.08.013
氧化苦参碱通过下调Toll样受体4抑制非小细胞肺癌细胞迁移和侵袭
Oxymatrine inhibits cell migration and invasion in non-small-cell lung cancer by down-regulating Toll-like receptor 4
摘要
Abstract
AIM:To investigate the therapeutic effect of oxymatrine on non-small-cell lung cancer(NSCLC) A549 cells and a xenograft mouse model,and to explore the underlying molecular mechanisms. METHODS:The effect of oxymatrine on the A549 cell viability was assessed by CCK-8 assay. After the A549 cells were treated with Toll-like re?ceptor 4(TLR4)stimulator lipopolysaccharide(LPS)and oxymatrine(5,10 and 15 mmol/L),the mRNA and protein ex?pression levels of TLR4 and myeloid differentiation factor 88(MyD88)were analyzed by RT-qPCR and Western blot,re?spectively. The migration and invasion abilities of the cells were measured by Transwell assay,and the mRNA and protein expression levels of matrix metalloproteinases-2(MMP-2),MMP-9 and vascular endothelial growth factor(VEGF)were also determined. A xenograft model in nude mice was utilized to evaluate the effect of oxymatrine on tumor growth. RE?SULTS:Oxymatrine inhibited the viability of A549 cells,decreased LPS-induced expression of TLR4,MyD88,MMP-2, MMP-9 and VEGF in A549 cells,and suppressed LPS-increased migration and invasion abilities of A549 cells. In the xe?nograft model,oxymatrine both reduced tumor growth and inhibited TLR4 expression in the tumor. CONCLUSION:Oxy?matrine exerts anti-tumor properties in NSCLC in vitro and in vivo by down-regulating the TLR4/MyD88 signaling pathway, suggesting that oxymatrine can be a potential therapeutic agent for NSCLC.关键词
氧化苦参碱/非小细胞肺癌/细胞迁移/细胞侵袭/Toll样受体4Key words
Oxymatrine/Lipopolysaccaride/Non-small-cell lung cancer/Cell migration/Cell invasion/Toll-like receptor 4分类
医药卫生引用本文复制引用
胡亚娥,杨萍,茅家慧..氧化苦参碱通过下调Toll样受体4抑制非小细胞肺癌细胞迁移和侵袭[J].中国病理生理杂志,2021,37(8):1438-1446,9.基金项目
Supported by the National Natural Science Foundation of China(No.81503304),Nantong Science and Tech?nology Plan Project(No.MS12015049),grants of 2019 Jiangsu Government Scholarship for Overseas Studies(No.JS-2019-255). （No.81503304）
