首页|期刊导航|世界中医药杂志（英文版）|Exploration of Bioactive Constituents and Immunoregulatory Mechanisms of a Hanshi-Yufei Formulation for Treating COVID-19
世界中医药杂志（英文版）2021，Vol.7Issue(3)：339-346,8.DOI:10.4103/2311-8571.321975
Exploration of Bioactive Constituents and Immunoregulatory Mechanisms of a Hanshi-Yufei Formulation for Treating COVID-19
Exploration of Bioactive Constituents and Immunoregulatory Mechanisms of a Hanshi?Yufei Formulation for Treating COVID?19
摘要
Abstract
Objective: The objective of this study was to characterize the chemical compounds of a Hanshi?Yufei formulation (HSYF; a modified formulation of a traditional Chinese medicine used for treating COVID?19) to elucidate the mechanism of action and to evaluate potential anti?inflammatory effects of HSYF. Materials and Methods: The chemical constituents of HSYF extract were characterized using UPLC?Q?TOF/MS. Subsequently, a set of TCM network pharmacology methods was applied to identify disease?associated genes and to predict target profiles and pharmacological actions associated with the constituents of HSYF. Then, the antiviral effects of HSYF on H1N1 were assessed in RAW264.7 cells using MTT assays. Expression levels of pro?inflammatory cytokines interleukin (IL)?6 and tumor necrosis factor (TNF)?α following infection of RAW264.7 cells with H1N1 were measured using an enzyme?linked immune sorbent assay (ELISA), and expression levels of inflammatory?related factors were detected using western blotting. Results: In total, 165 chemical constituents (including glycosides, tannins, volatile oils, amino acids, triterpenoids, polyphenols, phenylpropanoids, sesquiterpenes, alkaloids, and flavonoids, among others) were tentatively identified in HSYF. Network pharmacology demonstrated that HSYF can regulate immunomodulatory? and anti?inflammatory?related targets of multiple pathways through its active ingredients, suggesting potential anti?COVID?19 effects. Furthermore, cell viability assays and ELISA showed that HSYF significantly inhibited H1N1 replication in RAW64.7 cells and markedly reduced expression of pro?inflammatory cytokines TNF?α and IL?6 at the proteins level. Conclusions: The results of the present study help improve our understanding of the therapeutic effects of HSYF in COVID?19 treatment from multi?level perspectives.关键词
Anti‑inflammatory/COVID‑19/Hanshi‑Yufei prescription/immunoregulatoryKey words
Anti‑inflammatory/COVID‑19/Hanshi‑Yufei prescription/immunoregulatory引用本文复制引用
Wen?Long Wei,Wan?Ying Wu,De?An Guo,Shi?Fei Wu,Zhen?Wei Li,Hao?Jv Li,Hua Qu,Chang?Liang Yao,Jian?Qing Zhang,Jia?Yuan Li,Gao?Le Zhang..Exploration of Bioactive Constituents and Immunoregulatory Mechanisms of a Hanshi-Yufei Formulation for Treating COVID-19[J].世界中医药杂志（英文版）,2021,7(3):339-346,8.基金项目
This work was supported by the National Key R&D Program of China?(NO.2019YFC1711000),the National Natural Science Foundation of China?(NO.81530095,81673591),the Strategic Priority Research Program of the Chinese Academy of Sciences?(NO.XDA12020348),the National Standardization of Traditional Chinese Medicine Project?(NO.ZYBZH K LN 01),the Science and Technology Commission Foundation of Shanghai?(NO.15DZ0502800),and the Projects of Research and Develop Plan in the Key Field of Guangdong?(No 2020B1111110007). （NO.2019YFC1711000）
