南京林业大学学报（自然科学版）2021，Vol.45Issue(5)：10-18,9.DOI:10.12302/j.issn.1000-2006.202012046
基于SSR标记的丛生竹杂种鉴定、遗传分析和指纹图谱构建
SSR-based hybrid identification, genetic analyses and fingerprint development of hybridization progenies from sympodial bamboo ( Bambusoideae, Poaceae)
摘要
Abstract
[Objective] The study aims to accurately verify sympodial bamboo hybrids, clarify genetic relationships between hybrids and their parents, and develop applicable fingerprints. [Method]We screened, tested and analyzed 30 pairs of randomly selected published bamboo simple sequence repeat ( SSR) primers using hybrids derived from Bambusa multiplex × Dendrocalamus latiflorus and B. multiplex × B. chungii crosses. [Result]According to the results, six SSR primer pairs ( P8, P9, P18, P19, P20 and P27) were suitable for the hybrid identification and genetic analysis of the B. multiplex × D. latiflorus cross, which revealed that all 34 progenies were true hybrids ( 100％) . Six SSR primer pairs ( P2, P9, P10, P11, P20 and P28) were suitable for the hybrid identification and genetic analysis of the B. multiplex × B. chungii cross, which revealed that 54 of the 59 progenies were true hybrids (91.5％). Hybrids of the B. multiplex × D. latiflorus cross inherited more maternal alleles, while hybrids of the B. multiplex × B. chungii cross inherited more paternal alleles. The alleles detected using SSR primers were used to construct hybrid fingerprints of the two crosses for variety protection. [Conclusion] The SSR primers could be used to effectively verify sympodial bamboo hybrids as the progeny inherited alleles from different parents at varying probabilities in different crosses. In addition, the development of hybrid fingerprints could facilitate variety protection.关键词
丛生竹/杂交子代/SSR/杂种鉴定/遗传分析/指纹图谱Key words
sympodial bamboo/hybridization progenies/simple sequence repeat ( SSR )/hybrid identification/genetic analyses/fingerprint分类
农业科技引用本文复制引用
袁金玲,马婧瑕,钟远标,岳晋军..基于SSR标记的丛生竹杂种鉴定、遗传分析和指纹图谱构建[J].南京林业大学学报（自然科学版）,2021,45(5):10-18,9.基金项目
浙江省农业新品种选育重大专项(2021C02070-4). （2021C02070-4）
