广西医科大学学报2021，Vol.38Issue(9)：1639-1646,8.DOI:10.16190/j.cnki.45-1211/r.2021.09.001
姜黄素通过抑制HMGB1介导的自噬对棕榈酸诱导的卵巢颗粒细胞凋亡和类固醇生成能力的影响
Effect of curcumin on palmitic acid-induced apoptosis and steroid production of ovarian granulosa cells by inhibiting HMGB1-mediated autophagy
摘要
Abstract
Objective:To explore the effect of curcumin on the injury of ovarian granulosa cells induced by palmitic acid(PA) and its possible mechanism.Methods:Human ovarian granulosa cells were cultured in vitro.CCK-8 was used to de-tect cell viability.ELISA was used to detect estradiol (E2) con-tent in the cell supematant.RT-qPCR was used to detect the ex-pression of HMGB1,CYP19A and CYP11A mRNA.Flow cy-tometry was used to detect cell apoptosis.Western blotting was used to detect the expression of HMGB1,Beclinl,LC3B and P62 protein.Results:200 μmol/L or 400 μmol/L PA signifi-cantly decreased the cell viability,E2 content,and the expres-sion of CYP19A and CYP11A mRNA,while increased the apoptosis rate of cells (P＜0.05).Compared with the control group,the cell viability,E2 content,and the expressions of CYP19A,CYP11A and P62 in the PA group were significantly reduced,while the cell apoptosis rate,the expressions of HMGB1 and Beclinl,and LC3Ⅱ/LC3Ⅰ ratio were significantly elevated (P＜0.05).Compared with the PA group,the cell via-bility,E2 content,and the expressions of CYP19A,CYP11A and P62 in PA + curcumin (2.5 μmol/L or 5 μmol/L) group were increased,while the cell apoptosis rate,the expression of HMGB1 and Beclin1,and LC3Ⅱ/LC3Ⅰ ratio were evidently re-duced (P＜0.05).Overexpression of HMGB1 reversed the pro-tective effects of curcumin on the PA induced injuries of ovari-an granulosa cells and up-regulated the expressions of autopha-gy-related proteins (P＜0.05).Conclusion:Curcumin can at-tenuate PA-induced ovarian granulosa cell injury by inhibiting autophagy mediated by HMGB1.关键词
卵巢颗粒细胞/棕榈酸/姜黄素/高迁移率族盒蛋白1/自噬Key words
ovarian granulosa cells/palmitic acid/curcumin/high mobility group box protein 1/autophagy分类
医药卫生引用本文复制引用
廖源,王开举,李浩僡,陈惠萍,李选逸..姜黄素通过抑制HMGB1介导的自噬对棕榈酸诱导的卵巢颗粒细胞凋亡和类固醇生成能力的影响[J].广西医科大学学报,2021,38(9):1639-1646,8.基金项目
This study was funded by Scien-tific Research Project of Health Industry in Hainan Province (No.20A200353) and The National Natural Science Foundation of China (81803277). （No.20A200353）
