昆虫学报2021，Vol.64Issue(9)：1020-1030,11.DOI:10.16380/j.kcxb.2021.09.002
沙葱萤叶甲成虫不同夏滞育阶段的转录组分析
Analysis of the transcriptomes of Galeruca daurica(Coleoptera:Chrysomelidae) adults at different summer diapause stages
摘要
Abstract
[Aim]This study aims to explore the crucial genes and metabolic pathways involved in obligatory summer diapause in Galeruca daurica,a new pest with great outbreak in the Inner Mongolia grasslands,northern China.[Methods]Using RNA-Seq,we performed sequencing,transcriptional profiling and functional prediction for G.daurica adults at different summer diapause stages,i.e.pre-diapause (PD),diapause (D)and post-diapause (TD),and screened differentially expressed genes (DEGs) at different summer diapause stages based on the RNA-Seq data.The expression levels of ten DEGs screened based on the RNA-Seq data were verified by qPCR.[Results]A total of 202770198 clean reads from nine libraries were filtered,and 12078060 transcripts were assembled into 82292 unigenes with an average length of 783.59 bp and a N50 of 1545 bp.The 2395 (2119 up-regulated and 277 down-regulated) and 62 (59 up-regulated and 3 down-regulated) DEGs were identified in the D vs PD and TD vs D comparison groups,respectively.The KEGG analysis revealed that the glycolysis/gluconeogenesis and fatty acid biosynthesis pathways were significantly enriched in the D vs PD and TD vs D comparison groups,respectively.Additionally,many DEGs related to the Ca2+ signaling were differentially expressed during diapause.Finally,the expressional analysis result by qPCR for ten DEGs showed a high consistency between qPCR and RNA-Seq results.[Conclusion]The glycolysis/gluconeogenesis,fatty acid biosynthesis,and Ca2+ signaling pathways may play an important role in diapause regulation of G.daurica.This study establishes a foundation for future studies on the molecular mechanism underlying obligatory summer diapause in G.daurica.关键词
沙葱萤叶甲/夏滞育/专性滞育/RNA-SeqKey words
Galeruca daurica/summer diapause/obligatory diapause/RNA-Seq分类
生物科学引用本文复制引用
李艳艳,陈龙,李玲,谭瑶,庞保平..沙葱萤叶甲成虫不同夏滞育阶段的转录组分析[J].昆虫学报,2021,64(9):1020-1030,11.基金项目
国家自然科学基金项目(31760517) （31760517）
