数字中医药（英文）2021，Vol.4Issue(3)：202-213,12.DOI:10.1016/j.dcmed.2021.09.005
基于网络药理学探究"川芎-苏合香-冰片"组方挥发油治疗心绞痛作用机制及其对心肌受损大鼠的保护作用
Mechanism of volatile oil from Chuanxiong (Chuanxiong Rhizoma) - Suhexiang (Styrax) - Bingpian (Borneolum) in treat-ing angina pectoris based on network pharmacology and its pro-tective effects on myocardial damage in rats
摘要
Abstract
Objective To explore the pharmacodynamic material basis and mechanism of action of volatile oil from Chuanxiong (Chuanxiong Rhizoma) - Suhexiang (Styrax) - Bingpian (Borneo-lum) (hereinafter referred to as C-S-B volatile oil) in treating angina pectoris based on network pharmacology and to detect its protective effects against rat myocardial damage. Methods Gas chromatography-mass spectrometry (GC-MS) was used to determine the constituents of volatile oils from Chuanxiong (Chuanxiong Rhizoma), Suhexiang (Styrax), and Bingpian (Borneolum), and the targets of the three main con-stituents were found predicted and screened using the PharmMapper server, and GeneCards and CooLGeN databases. The STRING database and Cytoscape software were used to draw the protein-protein interaction (PPI) network, RStudio software was used to analyze Gene Ontology (GO) and Kyoto Encyclope-dia of Genome and Genome (KEGG) pathways, and Cytoscape software was used to construct the component-target-pathway-disease network. The rat model of myocardial injury was estab-lished by intraperitoneal injection of a large dose of isoprenaline hydrochloride. After continuous intervention with C-S-B volatile oil for 14 d, the ejection fraction (EF) and short axis shortening rate (FS) of the left ventricle were detected. The indices of myocardial damage were detected after hematoxylin-eosin (HE) staining. Results Fifteen volatile oil components from the C-S-B formula were identified. There are 470 targets of these volatile oil components and 401 angina-related genes. There are 28 core tar-gets, including CHRM4, ADRA1A, FGFR1, CHRM2, CYP2A6, CHRM5, CHRM1, CHRM3, HDAC2, and MPO, etc.. The results of the KEGG analysis indicated that the C-S-B formula probably in-terferes with the following pathways: neuroactive ligand-receptor interactions, calcium signaling, cytochrome P450 metabolism of heteropoietin, among others. The results of animal experiments showed that the C-S-B formula essential oil could significantly improve the following myocardial indices in rats with myocardial injury: EF, FS, left ventricular end-systolic diameter (LVIDs), left ventricular end-diastolic diameter (LVIDd), and stroke volume (SV), and all the differences were statistically significant (P < 0.01).Conclusion The mechanism of action of volatile oil components in the C-S-B formula in treating angina pectoris was analyzed us-ing multi-component, multi-target and multi-pathway systems, which has laid a foundation for further revealing its mechanism of action. Animal experiments have shown that the volatile oil of the C-S-B formula can improve EF, FS, and other indices of myocardial damage in a rat model, thus relieving myocardial damage caused by heart hyperactivity, improving cardiac func-tion, and protecting against myocardial damage.关键词
川芎/苏合香/冰片/挥发油/心绞痛/气相色谱-质谱/网络药理学/心肌受损/大鼠模型Key words
Chuanxiong (Chuanxiong Rhizoma)/Suhexiang (Styrax)/Bingpian (Borneolum)/Volatile oil/Angina pectoris/Gas chromatography-mass spectrometry (GC-MS)/Network pharmacology/Myocardial injury/Rat model引用本文复制引用
雷志强,王朝平,Abid Naeem,殷宁,曹美芳,罗晶..基于网络药理学探究"川芎-苏合香-冰片"组方挥发油治疗心绞痛作用机制及其对心肌受损大鼠的保护作用[J].数字中医药（英文）,2021,4(3):202-213,12.基金项目
We thank for the funding support from the Major Science and Technology Research and Development Special Project of Jiangxi Science and Technology Department (No. 20194ABC28009), and National Key Research and Development Plan (No. 2018YFC1706404). （No. 20194ABC28009）
