数字中医药（英文）2021，Vol.4Issue(3)：229-240,12.DOI:10.1016/j.dcmed.2021.09.007
白扁豆总皂苷降糖活性组分筛选及其功能评价
Screening and functional evaluation of the glucose-lowering active compounds of total saponins of Baibiandou (Lablab Semen Album)
摘要
Abstract
Objective To screen for α-glucosidase inhibitor active com-pounds in the total saponins of Baibiandou (Lablab Semen Al-bum) based on UHPLC-Q-Exactive Orbitrap MS technology and to evaluate its hypoglycemic activity in vivo. Methods Acarbose was used as the positive control, and the me-dian inhibitory concentration (IC50) was used as the evaluation index of α-glucosidase inhibitory activity to establish an in vitro α-glucosidase inhibition model. Further, UHPLC-Q-Exactive Orbit-rap MS technology was used to screen and identify the active compounds of α-glucosidase inhibitors in the total saponins of Baibiandou (Lablab Semen Album) in order to further verify the activity of the main active monomer and to perform homologous modeling and molecular docking of yeast-derived α-glucosidase and human-derived α-glucosidase, while the hypoglycemic activ-ity was evaluated in diabetic mice. Results This study successfully identified 15 compounds with potential α-glucosidase inhibitory activity, including Chikuset-susaponin IVa, from the total saponins of Baibiandou (Lablab Se-men Album). Simultaneously, we verified the activity of the main active monomer Chikusetsusaponin IVa, and showed that it has strong α-glucosidase inhibitory activity. The α-glucosidase inhib-itory concentration IC50 was (565.2 ± 1.026) μg/mL, and the IC50 of acarbose, which was lower than the positive control, was (706.6 ± 1.058) μg/mL. The docking energies of Chikusetsusapon-in IVa were – 6.1 and – 7.7 kcal/mol with yeast-derived α-glucosi-dase and human-derived α-glucosidase molecules, respectively. Both showed strong binding activity, and the levels of alanine aminotransaminase (ALT), aspartate aminotransaminase (AST), UREA, Creatinine (CREA), and cholesterol (CHO) were significantly decreased by Chikusetsusaponin IVa (P < 0.05). In addition, it could repair damaged liver and pancreas cells of dia-betic mice to some extent.Conclusion This study provides a basis for screening α-glucosi-dase inhibitors and structural modifications of the total saponins of Baibiandou (Lablab Semen Album).关键词
白扁豆/总皂苷/UHPLC-Q-Exactive Orbitrap MS/α-葡萄糖苷酶/分子对接/2型糖尿病小鼠/竹节参皂苷IVaKey words
Baibiandou (Lablab Semen Album)/Total saponins/UHPLC-Q-Exactive Orbitrap MS/α-Glucosidase/Molecular docking/Type 2 diabetic mice/Chikusetsusaponin IVa引用本文复制引用
韩君,郑钦方,方良子,黄小龙..白扁豆总皂苷降糖活性组分筛选及其功能评价[J].数字中医药（英文）,2021,4(3):229-240,12.基金项目
We thank for the funding support from the Program of the Educational Commission of Hunan Province of China(No.20B418). （No.20B418）
